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174,739 results

Showing: 10201 - 10240 of 174739 results

  1. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  5. mCherry-Nucleus-7

    Plasmid
    #55110
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mCherry-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  10. MAVS-Mito

    Plasmid
    #44556
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells
    Depositor
    Jonathan Kagan
    Article
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Fis1 chimeric protein (MAVS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa127-152 of Fis1
    Available Since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP160
    Mutation
    D10A;H840A nuclease-deficient
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48221-plasmid-map-sequence-id-69506

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  21. pGEX6P1-3xMBT_D355N

    Plasmid
    #46988
    Purpose
    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    Mutation aspartic acid 355 to asparagine (D355N);…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46988-plasmid-map-sequence-id-66963

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  22. CMV-CAR-GECO1

    Plasmid
    #45493
    Purpose
    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    CAR-GECO1
    Expression
    Mammalian
    Mutation
    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    45493-plasmid-map-sequence-id-315961

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Showing: 10201 - 10240 of 174739 results