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159,352 results

Showing: 10281 - 10320 of 159352 results

  3. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  7. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Article
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    44965-plasmid-map-sequence-id-352462

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  10. pYPQ203 (pMDC32-Ubi1)

    Plasmid
    #86207
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Maize ubiquitin 1 (ZmUbi1)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86207-plasmid-map-sequence-id-174284

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  12. pENN.AAV.CB7.CI.eGFP.WPRE.rBG

    Plasmid
    #105542
    Purpose
    AAV expression of EGFP from CB7 promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB7
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105542-plasmid-map-sequence-id-201063

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  15. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

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  18. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104992-plasmid-map-sequence-id-213507

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  25. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  29. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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  33. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid
    #79601
    Purpose
    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    d2GFP-Zeb1 3'UTR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79601-plasmid-map-sequence-id-398021

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  35. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  36. mCherry-Vinculin-23

    Plasmid
    #55159
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55159-plasmid-map-sequence-id-220874

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  37. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  39. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

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Showing: 10281 - 10320 of 159352 results