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179,521 results

Showing: 10361 - 10400 of 179521 results

  3. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73206-plasmid-map-sequence-id-139721

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  4. pGolt-mCherry / pGolt3-mCherry

    Plasmid
    #73297
    Purpose
    Visualization of Dendritic Golgi Satellite
    Depositor
    Michael Kreutz
    Publication
    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    Tags
    ER export and mCherry
    Expression
    Mammalian
    Promoter
    CMV immediate early promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73297-plasmid-map-sequence-id-135341

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  10. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Boris Fehse
    Publication
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Mutation
    changed Asp at position 249 + 250 to Val
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60488-plasmid-map-sequence-id-96845

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  13. pmCherryC2-Gephyrin P1

    Plasmid
    #68820
    Purpose
    mCherry tagged Geph for mammalian expression
    Depositor
    Shiva Tyagarajan
    Publication
    Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gephyrin (Gphn Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    silent mutations on internal EcoRI site. Gephyrin…
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68820-plasmid-map-sequence-id-417807

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  14. GCGR-Tango

    Plasmid
    #66291
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCGR (GCGR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. IRES-GFP-AXL-KD

    Plasmid
    #65498
    Purpose
    Expresses kinase dead AXL in mammalian cells with cistronic EGFP
    Depositor
    Aaron Meyer
    Publication
    Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tyrosine-protein kinase receptor UFO (AXL Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K567R
    Promoter
    CMV
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65498-plasmid-map-sequence-id-115389

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  23. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Publication
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61395-plasmid-map-sequence-id-101662

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  27. PDIA3-bio-His

    Plasmid
    #52070
    Purpose
    Expresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDIA3 (PDIA3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52070-plasmid-map-sequence-id-270198

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  30. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  33. mCherry-Nucleus-7

    Plasmid
    #55110
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mCherry-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  34. mCherry-PCNA-19-SV40NLS-4

    Plasmid
    #55117
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Publication
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58540-plasmid-map-sequence-id-90629

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  36. mCherry-CENPA-C-22

    Plasmid
    #55015
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CENPA (CENPA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55015-plasmid-map-sequence-id-492811

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  38. MAVS-Mito

    Plasmid
    #44556
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells
    Depositor
    Jonathan Kagan
    Publication
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Fis1 chimeric protein (MAVS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa127-152 of Fis1
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 10361 - 10400 of 179521 results