We narrowed to 43,548 results for: cha;
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Plasmid#114180PurposeORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).DepositorInsertORAI1-YFP V107M (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationV107MPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-HsPcdh21-Myc-Flag-His
Plasmid#210818PurposeMammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)DepositorInsertProtocadherin 21 (CDHR1 Human)
UseTagsMyc, 3x Flag, 10x HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG1(E793K)
Plasmid#79433PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cellsDepositorInsertBIG1 (ARFGEF1 Human)
UseTagsHA, His6, and XpressExpressionMammalianMutationchanged Glutamic acid 793 to LysinePromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)
Plasmid#223770PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI2d (WIPI2 Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationR108E/R125EPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5-4x6T
Plasmid#196419PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV and Cre/Lox; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 1)-myc
Plasmid#62845PurposeMammalian Expression of myc-tagged Opa1 isoform1DepositorInsertmmOPA1isoform1-myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRTagsExpressionMammalianMutationPromotershort human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple
Plasmid#214417PurposePeroxisome localization of PMP34 fused to mAppleDepositorInsertPMP34 (SLC25A17 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A_deltaSIV
Plasmid#145771Purposeexpresses human Nav1.5 lacking the SIV motif in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
UseTagsnoneExpressionMammalianMutationStop codon at Serine 2013PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX5_NCOA5
Plasmid#205885PurposeExpress mEGFP-tagged fusion protein, PAX5_NCOA5 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-BacMam-4x-PyOtR
Plasmid#217364PurposeExpresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmidDepositorInsert4 copies of pyrrolysyl-tRNA mutant PyOtR
UseBaculoviralTagsExpressionInsect and MammalianMutationPromoterU6Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR(A2.1)-mCherry
Plasmid#204869PurposeExpresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA mutant "A2.1"
UseAAVTagsExpressionMammalianMutationU25C; A3G, A4G, T65C, T66C, T67TPromoterU6Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_MTF1_SF3A3
Plasmid#205861PurposeExpress mEGFP-tagged fusion protein, MTF1_SF3A3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-full length LIC1
Plasmid#74601Purposeexpresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 FL
Plasmid#58893PurposeExpresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.DepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationL722FPromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai1
Plasmid#73561PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertNM_032790.3 (ORAI1 Human, Synthetic)
UseTagsG-GECO1ExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-K121M
Plasmid#64877Purposelentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationK121M, a mutation in the conserved ATP-binding si…PromoterEF1alphaAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DY2230AA
Plasmid#64878Purposelentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationD2330A,Y2331A, in the DNA polymerase domain (POL)…PromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD Cas13d P2A mCherry
Plasmid#155183PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherryDepositorInsertCasRx
UseUnspecified; Piggybac transposonTagsNLS and P2A mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL-C145A
Plasmid#160279PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC145APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS
Plasmid#164565PurposeSARS-CoV-2 Spike protein (S-GSAS variant)DepositorInsertSpike (S-GSAS variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX
Plasmid#169729PurposeBacterial expression of N-terminally 6His tagged cDAXXDepositorInsertDAXX (DAXX Human)
UseTags6 HisExpressionBacterialMutationPromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V 2014 EBOV + Mucin-Like Domain
Plasmid#86016PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged alanine 82 to valinePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
TPC1(ΔN69)-mCherry
Plasmid#135184PurposeTPC1 with truncated N-terminus, tagged with mCherry on C terminus.DepositorInsertTPC1 (Tpcn1 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationDeleted amino acids 1-69PromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
JWW-2 human chimeric monoclonal antibody
Plasmid#66749PurposeExpresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPVDepositorInsertsJWW-2 Heavy Chain
JWW-2 Light Chain
UseTagsExpressionMammalianMutationPromotermEF1 and rEF1Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWG-dCas9-VPR
Plasmid#63802PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vectorDepositorInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionInsectMutationPromoteract5cAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST MTFR1L-V5
Plasmid#69822PurposeExpresses MTFR1L-V5 in mammalian cellsDepositorInsertMitochondrial fission regulator 1-like (MTFR1L Human)
UseRetroviralTagsv5ExpressionMammalianMutationPromotercmvAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TPC2(ΔN49)-mCherry
Plasmid#135185PurposeTPC2 with truncated N-terminus, tagged with mCherry on C terminus.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationDeleted amino acids 1-49PromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-β′-COPI-WD40
Plasmid#180753PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).DepositorInsertβ′-COPI-WD40 (SEC27 Budding Yeast)
UseTagsN-terminal strep tag, His-tag, and SUMO tagExpressionBacterialMutationEncodes residues 1-301; deleted 302-889; mutated …PromoterT7Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-DsRed-MST-Kv2.1
Plasmid#131707PurposeExpresses Kv2.1in mammalian cellsDepositorInsertKCNB1 (KCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/D614G
Plasmid#164570PurposeSARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)DepositorInsertSpike (S-RRAR-D614G variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationD614GPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
UseTags6X Histidine Tag, TEV cleavage siteExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-WPRE
Plasmid#44358DepositorInsertRK-GFP (MAPK14 Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2557-2853)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR(NGS-6)-mCherry
Plasmid#217363PurposeExpresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA mutant, "NGS-6"
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LZRS-Flag_EZH2_ER
Plasmid#26111DepositorInsertEZH2 (EZH2 Human)
UseRetroviralTagsER and FLAGExpressionMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only