We narrowed to 28,961 results for: Tat
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Plasmid#138188Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only
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pLV_iRFP_P2AT2A_mScarlet-I_Myl9
Plasmid#172474PurposeMammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.DepositorInsertsUseLentiviralTagsmScarlet-IExpressionMammalianPromoterEF1alpha (with UCOE) and none (same transcript as…Available SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shSNAI1
Plasmid#115467PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167971PurposeTp63 reporter targeting repair templateDepositorInsertTp63 (TP63 Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianPromoterCAGAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX317-MBNL2
Plasmid#115444PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V678F-VSV
Plasmid#139349PurposeExpression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362F-V678FPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E
Plasmid#79562Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Delete
Plasmid#159143PurposeExpression and protien purification of a mutated RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
ExpressionBacterialMutationamino acid 2-456, Thr127-Glu233 mutated to GPPGAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC-SUR-YR
Plasmid#61768PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).DepositorInsertsSur gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…PromoterMagnaporthe oryzae ILV promoterAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-FAF1-Strep-HA
Plasmid#113486PurposeExpression of human FAF1 with C-terminal strep-HA tagDepositorInsertFAF1 (FAF1 Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (NC-flag)
Plasmid#156418PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Chicken ArcLight A173
Plasmid#53615PurposeGenetically encoded voltage sensor ArcLight with improved kineticsDepositorInsertChicken ArcLight-A173
ExpressionMammalianMutationGg-VSP contains an R153Q mutation and an amino ac…PromoterCMVAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CamKII-ADAM23-pHluorin
Plasmid#185539PurposeExpresses ADAM23-pHluorin under the CamKII promoterDepositorAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E(D556A)
Plasmid#79564Purposeexpression of CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRECLV103_PGBD5_B
Plasmid#168053PurposeLentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1DepositorAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Y276H PHYB-CAAX
Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40
Plasmid#105983PurposeThis AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInsertFLEX-SYFP2-POST-T2A-dTomato.FLEX
UseAAV and Cre/LoxTagsSYFP2-POST, T2A-dTomato, and mycExpressionMammalianPromoterhuman Synapsin 1Available SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ETV1
Plasmid#98623PurposeGateway entry TOPO TA cloning vector for ETV1DepositorInsertETV1 (Etv1 Mouse)
UseTopo ta cloning vectorAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA USP7
Plasmid#131252PurposeMammalian expression of N-terminally Myc-tagged DW164AA USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationContains a point mutation that converts USP7 aspa…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
TagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contai…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_WT_ADRB2_LucReporter
Plasmid#129788Purposeencodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G469A
Plasmid#131711PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7 C450A
Plasmid#104628Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutationDepositorInsertCaspase-3 (CASP3 Human)
TagsHis and LOV2ExpressionBacterialMutationC450A (dark-locked and light-insensitive mutation)PromoterT7Available SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK4
Plasmid#98617PurposeGateway entry TOPO TA cloning vector for ELK4DepositorInsertELK4 (Elk4 Mouse)
UseTopo ta cloning vectorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Ataxin3-Strep-HA
Plasmid#113496PurposeExpression of human Ataxin-3 with C-terminal strep-HA tagDepositorInsertAtaxin-3 (ATXN3 Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
FOR human OCT4 TALEN
Plasmid#52412PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD NG NN NN NN HD NG HD NG HD HD HD NI NG
UseTALENPromoterCAGGSAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
LMPd Ametrine Got2 shRNA#3
Plasmid#220593PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot2 shRNA (Got2 Mouse)
UseRetroviralAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-ND-Cdt1-mCherry
Plasmid#193761PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-Elf5 gRNA
Plasmid#128836PurposegRNA for targeting mouse Elf5 loci using CRISPR-cas techniqueDepositorInsertmElf5 gRNA (Elf5 Mouse)
UseCRISPRAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shMBNL1-9155
Plasmid#115460PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS
Plasmid#162613PurposeAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
ExpressionMammalianMutation1-155-I152LAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-V5H6-mPCSK3 D153A
Plasmid#122681PurposeExpresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
ExpressionMammalianMutationchanged Aspartic Acid 153 to AlanineAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-S1977P
Plasmid#64876Purposelentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationS1977P, mimicking the chaos1 mutationPromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC16A10
Plasmid#131946PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC16A10 (SLC16A10 Human)
ExpressionMammalianAvailable SinceOct. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits