159,132 results

1. pPB-cT3G-cERP2-NELFE

   Plasmid

   #222559

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   NELFE (NELFE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLENTI_RPS3_Keima

   Plasmid

   #127140

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS3_Keima (RPS3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-SFPQ

   Plasmid

   #166960

   Purpose

   Expresses FLAG-tagged SFPQ protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-HsPcdh21-mNeonGreen

   Plasmid

   #210819

   Purpose

   Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT423

   Plasmid

   #139382

   Purpose

   Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Constitutively expressed dCas9, LacI and TetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P1

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. anxA1-GFP

   Plasmid

   #107194

   Purpose

   Expresses porcine annexin A1-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A1
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

   Plasmid

   #163030

   Purpose

   Expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Alb-AAT

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lamp1-mCherry-RUSH

   Plasmid

   #202799

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   Lamp1 (Lamp1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. mApple-TNFa-RUSH

    Plasmid

    #166902

    Purpose

    RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK CA MLCK

    Plasmid

    #84647

    Purpose

    Lentiviral expression of doxycycline-inducible constitutively active MLCK and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

    Insert

    MLCK (MYLK Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Constitutive Active

    Promoter

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. paGFP-Omp25

    Plasmid

    #69598

    Purpose

    expresses GFP-labeled mitochondrial outer membrane protein 25

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    paGFP-Omp25 (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    photoactivatable GFP fused to rat Omp25 C-terminus

    Promoter

    CMV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-GGamma1-GFP2

    Plasmid

    #140989

    Purpose

    Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma1-GFP2 (GNGT1 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PME-1 pcDNA-StrepIII

    Plasmid

    #119292

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1

    Depositor

    Jukka Westermarck

    Article

    Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

    Insert

    PPME1 (PPME1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF1577

    Plasmid

    #143133

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    AHR (AHR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSGV Mouse Acceptor PGK NGFR

    Plasmid

    #128482

    Purpose

    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Rab11a-7

    Plasmid

    #56444

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCMV/myc/mito-hOGG1

    Plasmid

    #18706

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 . 6():235.

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pCMV mouse Dscam

    Plasmid

    #18737

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

    Insert

    Dscam (Dscam Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAd5-B1

    Plasmid

    #122551

    Purpose

    Block 1 for AdenoBuilder genome assembly

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 1-3759
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF0276

    Plasmid

    #142386

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC1-V16-eGFP

    Plasmid

    #122033

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hCMV-IE1:RedF

    Plasmid

    #118064

    Purpose

    Transcriptional unit for constitutive expression of RedF in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:RedF:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3- c-terminal 6xMyc

    Plasmid

    #128023

    Purpose

    Mammalian expression vector with C terminal 6xMyc tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    6xMyc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid

    #183252

    Purpose

    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    anti-CD19 scFvFc (FMC-63)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. myc-tagged NRP1 (human)

    Plasmid

    #213068

    Purpose

    Express myc-NRP1 in mammalian cells

    Depositor

    Yoav Henis

    Insert

    neuropilin 1 (NRP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal myc tag was introduced by a three-step…

    Promoter

    T7

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FUW-TetO-Sox9

    Plasmid

    #41080

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid

    #177308

    Purpose

    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid

    #66707

    Purpose

    expresses a single gRNA and VP64-dCas9-VP64

    Depositor

    Charles Gersbach

    Inserts

    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hU6 and hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA6 MCV LTco

    Plasmid

    #40200

    Depositor

    Patrick Moore

    Article

    Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge