Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 18,127 results for: Mut

Showing: 11721 - 11760 of 18127 results

  1. pCMT-flp

    Plasmid
    #67274
    Purpose
    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis
    Depositor
    Mark Liles
    Article
    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-
    Insert
    tetA, flp, repA101, oriR101
    Use
    Flp recombinase plasmid, temperature sensitive
    Tags
    His-SUMO (flp)
    Expression
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67274-plasmid-map-sequence-id-115886

    Enlarge

  2. pVS2

    Plasmid
    #33144
    Depositor
    Michael Rosbash
    Article
    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.
    Inserts
    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    Use
    Tags
    Expression
    Yeast
    Mutation
    GFP is mutated such that amino acids 64-73 (FSYGV…
    Promoter
    bidirectional GAL1-GAL10 promoter
    Available Since
    Dec. 20, 2011
    Availability
    Academic Institutions and Nonprofits only

  3. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

    Enlarge

  6. pFP.E227

    Plasmid
    #138249
    Purpose
    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P17_up1691, P20_992, and iP16_3622
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138249-plasmid-map-sequence-id-273529

    Enlarge

  15. AICSDP-166:CDH1-mEGFP

    Plasmid
    #193921
    Purpose
    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1
    Depositor
    Allen Institute for Cell Science
    Insert
    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    Use
    AAV and CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193921-plasmid-map-sequence-id-485363

    Enlarge

  16. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50455-plasmid-map-sequence-id-214301

    Enlarge

  18. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

    Enlarge

  31. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

    Enlarge

  36. pCDNA3-LOV-VP16

    Plasmid
    #42499
    Purpose
    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Article
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Insert
    FKF1 (N-LOV, G128D mutant) fused with VP16
    Use
    Tags
    F-type VP16 activation domain and HA tag
    Expression
    Mammalian
    Mutation
    G128D 1-168aa
    Promoter
    CMV
    Available Since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    42499-plasmid-map-sequence-id-57336

    Enlarge

  38. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Use
    Tags
    E6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

    Enlarge

Showing: 11721 - 11760 of 18127 results