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Showing: 101 - 150 of 1842 results

  6. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Article
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107939-plasmid-map-sequence-id-207466

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  7. pJH4203

    Plasmid
    #200160
    Purpose
    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    twk-40 (twk-40 Nematode)
    Use
    Tags
    GFP
    Expression
    Worm
    Mutation
    Promoter
    Prig-3
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200160-plasmid-map-sequence-id-413719

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  8. pJJR83

    Plasmid
    #75028
    Purpose
    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Article
    Ramalho et al Development. 2020 Jul 22;147(14):dev1880
    Insert
    mCherry^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized mCherry
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75028-plasmid-map-sequence-id-141918

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  9. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163095-plasmid-map-sequence-id-320590

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  10. pJL046

    Plasmid
    #198807
    Purpose
    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198807-plasmid-map-sequence-id-390993

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  12. pJJS001

    Plasmid
    #188324
    Purpose
    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::GFP::Linker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188324-plasmid-map-sequence-id-370743

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  17. pCZGY2850

    Plasmid
    #87758
    Purpose
    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
    Depositor
    Andrew Chisholm
    Article
    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10
    Insert
    Pcol-19-PH-miniSOG(Q103L)
    Use
    Tags
    PH
    Expression
    Worm
    Mutation
    Q103L
    Promoter
    Pcol-19
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87758-plasmid-map-sequence-id-174819

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  21. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pJR021

    Plasmid
    #198814
    Purpose
    Variant of yellow fluorescent protein for transient gene expression
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::destabilizedYFP::let-838 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    YFP with destabilized signal from mouse ornithine…
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198814-plasmid-map-sequence-id-391694

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  29. pAS1-rab3P::his-24::mNeptune

    Plasmid
    #124350
    Purpose
    Nuclear Localized Pan-Neuronal mNeptune
    Depositor
    Andrew Leifer
    Insert
    rab-3, mNeptune (rab-3 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124350-plasmid-map-sequence-id-241971

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  31. pJJS012

    Plasmid
    #188335
    Purpose
    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mTagBFP2::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188335-plasmid-map-sequence-id-370742

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  34. pLT 653

    Plasmid
    #59996
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59996-plasmid-map-sequence-id-110769

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  36. pHJL4

    Plasmid
    #199379
    Purpose
    Encodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7f::SL2::mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199379-plasmid-map-sequence-id-390947

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  42. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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  44. pDD362

    Plasmid
    #137023
    Purpose
    SapTrap HaloTag-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized Halo Tag
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized Halo Tag
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137023-plasmid-map-sequence-id-274041

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  49. pCC324

    Plasmid
    #110165
    Purpose
    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    R61K change made in ERK-KTR CDS
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110165-plasmid-map-sequence-id-235338

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Showing: 101 - 150 of 1842 results