163,444 results

1. pCI-Caspase 1

   Plasmid

   #41552

   Depositor

   Kate Fitzgerald

   Article

   Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate-early enhancer/promoter

   Available Since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-KIT-V5/HIS

   Plasmid

   #201986

   Purpose

   expression of human KIT receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   KIT receptor tyrosine kinase (KIT Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

   Plasmid

   #204579

   Purpose

   lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

   Plasmid

   #34877

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Peft-3 GFP H2B tbb-2 UTR
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   S65C

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Feb. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hCas9

   Plasmid

   #51142

   Purpose

   Expresses hCas9 under the CAG promoter for CRISPR

   Depositor

   Izuho Hatada

   Article

   Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

   Insert

   hCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGGDestTol2LC-5sgRNA

   Plasmid

   #64243

   Purpose

   destination vector for five U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEGFP-Y705F-STAT3

    Plasmid

    #71445

    Purpose

    Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Y705F

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

11. CL20_mEGFP_NUP98_KDM5A

    Plasmid

    #205872

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_KDM5A (NUP98, KDM5A Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GW1-Peredox-mCitrine

    Plasmid

    #32386

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50464

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-HA-Ubiquitin-K63

    Plasmid

    #17606

    Purpose

    Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K63 only, other lysines mutated to arginines. Enh…

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pfmh-hCry1

    Plasmid

    #25843

    Depositor

    Aziz Sancar

    Insert

    human Cryptochrome 1 (CRY1 Human)
    

    Use

    Tags

    flag, his, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) Drp1

    Plasmid

    #34706

    Depositor

    David Chan

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge