Addgene

We narrowed to 4,068 results for: PRS

Showing: 1601 - 1650 of 4068 results

Results per page:

Sort results by:

pSUMO-LIC-CHIKV-nsP2h

Plasmid

#127935

Purpose

Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

Depositor

Dahai Luo

Article

Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

Insert

CHIKV nsP2h

Use

Tags

His6-Sumo-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

Plasmid

#119059

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

E1b minimal promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP Rab4

Plasmid

#99018

Purpose

Localization and visualization of trafficking protein Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 (Rab4 Fly, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL119

Plasmid

#21909

Depositor

Maria Castro

Article

Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

Insert

hCMV promoter expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
YFP Rab4DN

Plasmid

#99019

Purpose

Localization and visualization of the dominant negative form of Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 DN (Rab4 Fly, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

S22N Dominant negative

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP dsRed Rab7 WT

Plasmid

#44824

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL711

Plasmid

#140381

Purpose

Expresses OtrR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-OtrR-T

Use

Tags

TEV-6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL713

Plasmid

#140383

Purpose

Expresses CtcS protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CtcS-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP YFP Rab4 DN

Plasmid

#37691

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pJBL719

Plasmid

#140389

Purpose

Expresses QacR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-QacR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL721

Plasmid

#140391

Purpose

Expresses TtgR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TtgR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-myr-eGFP-SV40pA-FKF

Plasmid

#119866

Purpose

Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-myr-tdTomato-SV40pA-FKF

Plasmid

#119867

Purpose

Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hPlekhm1-C/pIRES puro Glue (S778-A1056)

Plasmid

#73456

Purpose

Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab3 WT

Plasmid

#37687

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab10 DN

Plasmid

#37709

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T23N (dominant negative)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFFV-Cx43

Plasmid

#69442

Purpose

mammalian expression of rat Cx43

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

Insert

Cx43 (Gja1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFusX4-35

Plasmid

#63444

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NI NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab10 CA

Plasmid

#37710

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q68L (constitutive active)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab7 WT

Plasmid

#37699

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO

Plasmid

#73448

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Article

Yang et al J Cell Physiol. 2008 May;215(2):497-505.

Insert

Ocstamp (Ocstamp Mouse)

Use

Tags

V5-6xHis

Expression

Mammalian

Mutation

N162D

Promoter

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP YFP Rab18 WT

Plasmid

#37717

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pFusX1-31

Plasmid

#63249

Purpose

Encodes RVD positions 1-3 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab8 WT

Plasmid

#37705

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (H987-end)

Plasmid

#73458

Purpose

Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-Q986)

Plasmid

#73457

Purpose

Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

Plasmid

#73455

Purpose

Express human middle part (Q513-E784) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab6 WT

Plasmid

#37696

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab18 DN

Plasmid

#53474

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

S19N

Promoter

GAL4

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab18 CA

Plasmid

#37719

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

A64L (constitutive active)

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pFusX4-31

Plasmid

#63440

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab1 WT

Plasmid

#37681

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab1 (Rab1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab3 WT

Plasmid

#44820

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Promoter

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab14 WT

Plasmid

#37714

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab14 (Rab14 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pEF Itk Y511F myc

Plasmid

#20709

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk Y511F (Itk Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Y511F.

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
p5’UTR-ORF1

Plasmid

#190566

Purpose

Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1

Use

For dna propagation only

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-myr-Citrine-SV40pA-FKF

Plasmid

#119865

Purpose

Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFusX4-41

Plasmid

#63549

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NN NI

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 1601 - 1650 of 4068 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests