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We narrowed to 2,111 results for: D10

Showing: 1651 - 1700 of 2111 results

  1. TFORF2360

    Plasmid
    #142047
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF641 (ZNF641 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142047-plasmid-map-sequence-id-395669

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  17. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  18. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  20. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  23. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  25. pcDNA3-Flag-Tet1 CD Mut

    Plasmid
    #72218
    Purpose
    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 1 (Tet1 Mouse)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET1 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    Use
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64929-plasmid-map-sequence-id-109691

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  29. TFORF0725

    Plasmid
    #143071
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143071-plasmid-map-sequence-id-399051

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  36. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Use
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  39. TFORF0724

    Plasmid
    #143070
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143070-plasmid-map-sequence-id-398863

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  42. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  49. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Article
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    131124-plasmid-map-sequence-id-257454

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Showing: 1651 - 1700 of 2111 results