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Showing: 1701 - 1750 of 12446 results

  6. pLV-BDNF-pHluorin

    Plasmid
    #187193
    Purpose
    To express the mouse BDNF tagged with the FP pHluorin
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF (Bdnf Mouse)
    Use
    Lentiviral
    Tags
    pPHluorin
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    187193-plasmid-map-sequence-id-373713

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  8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  9. pAAV-hSyn-GRAB_NTS1.0

    Plasmid
    #180006
    Purpose
    Expresses the neurotensin sensor GRAB_NTS1.0 in neurons
    Depositor
    Yulong Li
    Insert
    neurotensin sensor GRAB_NTS1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    180006-plasmid-map-sequence-id-358494

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  11. pcDNA3.1 AKT3 HA (CT-gfp)

    Plasmid
    #27293
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B gamma (Akt3 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Y473F
    Promoter
    Available since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

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  17. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid
    #122056
    Purpose
    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P
    Depositor
    Stephen Tapscott
    Article
    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do
    Insert
    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    MyoD chimeric protein substituted with the NeuroD…
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122056-plasmid-map-sequence-id-235399

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  18. PERK 7: PERK.K618A.9E10.pBABE

    Plasmid
    #21820
    Depositor
    David Ron
    Insert
    PERK K618A (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    kinase dead K618A mutant
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21820-plasmid-map-sequence-id-10016

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  20. Lazarus Gata 2 flag

    Plasmid
    #80137
    Purpose
    retroviral expression of Gata2-Flag
    Depositor
    Ellen Rothenberg
    Insert
    Gata2 (Gata2 Mouse)
    Use
    Retroviral
    Tags
    FLAG and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    80137-plasmid-map-sequence-id-157353

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  24. eGFP-proα2(I)-G610C

    Plasmid
    #119827
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119827-plasmid-map-sequence-id-233848

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  28. pEGFP-mNFM

    Plasmid
    #32909
    Purpose
    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M
    Depositor
    Anthony Brown
    Article
    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):
    Insert
    EGFP-mouse NFM fusion (Nefm Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32909-plasmid-map-sequence-id-38896

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  31. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid
    #159903
    Purpose
    Mutagenesis of Oprk1
    Depositor
    Larry Zweifel
    Insert
    Oprk1 (Oprk1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159903-plasmid-map-sequence-id-317307

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  41. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Article
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  42. pmIL-6 mut AP-1+C/EBP

    Plasmid
    #61292
    Purpose
    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated both AP-1 binding site from TGAGTCT to TG…
    Promoter
    IL-6 promoter with mutant AP-1 and C/EBP binding …
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1701 - 1750 of 12446 results