We narrowed to 2,195 results for: D10

1. pCAG R2pH-LAMP1-3xFLAG

   Plasmid

   #157940

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Tags

   3XFLAG, mCherry, and pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Chick beta actin pomoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK FKBP12(F36V)-OGT (Neo)

   Plasmid

   #154294

   Purpose

   Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag-Tet1 CD Mut

   Plasmid

   #72218

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-CR029

   Plasmid

   #234041

   Purpose

   expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   evoCDA1-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   blast

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CR102

   Plasmid

   #234043

   Purpose

   expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0725

   Plasmid

   #143071

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFKB2 (NFKB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Str-KDEL-SBP-EGFP-LAMP1

    Plasmid

    #222317

    Purpose

    Synchronize the trafficking of LAMP1 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CR033

    Plasmid

    #234042

    Purpose

    expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

    Depositor

    Alexander Marson

    Article

    Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

    Insert

    TadA8e(V106W)-Cas9(D10A)-P2A-blast
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-eNme2-T.2-ABE8e

    Plasmid

    #185669

    Purpose

    Expresses eNme2-T.2-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2-hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #225999

    Purpose

    To establish stable dCas9-KRAB knock-in line

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    humanized dCas9-KRAB T2A Puro
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_eA3A T31A-NG-nCas9

    Plasmid

    #163539

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-TRE3G-FNLS-PGK-Puro

    Plasmid

    #110847

    Purpose

    Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    TRE3G

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hu-TLR4 cDNA wt pDEST40

    Plasmid

    #42646

    Purpose

    destination vector for expression of human TLR4 in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 (TLR4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

23. TFORF0724

    Plasmid

    #143070

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFKB2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cbh_v5 AAV-saCBE N-terminal

    Plasmid

    #137182

    Purpose

    AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE N-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Str-KDEL_SBP-EGFP-LAMP2

    Plasmid

    #222321

    Purpose

    Synchronize the trafficking of LAMP2 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Str-KDEL_SBP-mCherry-LAMP1

    Plasmid

    #222318

    Purpose

    Synchronize the trafficking of LAMP1 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGFP-mTet1s pc3901

    Plasmid

    #201700

    Purpose

    Expresses of GFP-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LLP457 pGK-dCas9-Suntag-BFP

    Plasmid

    #100957

    Purpose

    BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA and BFP

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139094

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONOR223 RpH-LAMP1-3xFLAG

    Plasmid

    #163019

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEX-SP-YFP-STIM1(23-685)

    Plasmid

    #18857

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP is inserted between residues 1-23 (signal pep…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LAMP-mChF-AIP

    Plasmid

    #61524

    Purpose

    Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEX-SP-YFP-STIM1(D76A)

    Plasmid

    #18859

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D76A mutation - changed aspartate 76 to alanine. …

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

    Plasmid

    #163554

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Str-STIM1-NN_neomycin

    Plasmid

    #65311

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Use

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

    Plasmid

    #163561

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-2X-P2A-Puro

    Plasmid

    #110840

    Purpose

    Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-2X
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A and 2 tandem NLS sequences in the XTEN linker

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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42. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

    Plasmid

    #134611

    Purpose

    Mammalian expression of mouse KIF5A 378-1027

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. mCherry-CAD

    Plasmid

    #73566

    Purpose

    Soluble red fluorescent activator of Orai1 channels

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
    

    Use

    Tags

    6x His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLenti-gfaABC1D-monSTIM1

    Plasmid

    #161797

    Purpose

    Lentiviral vector expressing monSTIM1 in astrocytes.

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    gfaABC1D

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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