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Showing: 1751 - 1800 of 8550 results

  5. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid
    #125256
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection
    Depositor
    Roger D. Kamm
    Article
    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi
    Insert
    hChR2(H134R)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-forward
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    125256-plasmid-map-sequence-id-245091

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  6. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73022
    Purpose
    Luminescent and fluorescent dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73022-plasmid-map-sequence-id-136024

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  14. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

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  25. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid
    #47457
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Use
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47457-plasmid-map-sequence-id-69629

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  28. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73032
    Purpose
    Express fluorescent and luminescence dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73032-plasmid-map-sequence-id-136029

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  29. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  35. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #108274
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108274-plasmid-map-sequence-id-262430

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  41. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

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  45. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Article
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    50834-plasmid-map-sequence-id-76270

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  46. pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid
    #126080
    Purpose
    sCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)-eYFP
    Use
    AAV
    Tags
    eYFP
    Expression
    Mutation
    H134R
    Promoter
    Ef1a
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    126080-plasmid-map-sequence-id-245075

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Showing: 1751 - 1800 of 8550 results