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20,146 results

Showing: 11431 - 11460 of 20146 results

  1. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  4. pMpGWB337

    Plasmid
    #68061
    Purpose
    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter
    Depositor
    Takayuki Kohchi
    Article
    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68061-plasmid-map-sequence-id-487277

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  6. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid
    #128606
    Purpose
    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    Expression
    Mammalian
    Promoter
    Pcmv
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128606-plasmid-map-sequence-id-254156

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  18. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  21. LeGO-dKatushka2

    Plasmid
    #85214
    Purpose
    Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter
    Depositor
    Boris Fehse
    Article
    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1
    Insert
    dKatushka2
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    BsrGI site removed at 5' end, BsrGI site ins…
    Promoter
    SFFV
    Available Since
    Jan. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    85214-plasmid-map-sequence-id-164348

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  22. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  28. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Article
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

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Showing: 11431 - 11460 of 20146 results