163,445 results

1. pBiFC-VN155(I152L)

   Plasmid

   #27097

   Depositor

   Chang-Deng Hu

   Article

   Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

   Insert

   VN155(I152L)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   I152L

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-PS9

   Plasmid

   #177944

   Purpose

   Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-32a(+)-TGFB1m

   Plasmid

   #120286

   Purpose

   Produce human recombinant mTGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β1
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promotor

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209666

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His-Cre

   Plasmid

   #123128

   Purpose

   Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLminP_Luc2P_RE39

   Plasmid

   #90379

   Purpose

   PPARg - PPARg homodimer gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   RELB

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mEGFP-HRas

   Plasmid

   #18662

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-Intersectin Long

   Plasmid

   #47395

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCW-HA-hRb-WT-puro

   Plasmid

   #212672

   Purpose

   Express tagged hRb

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPJ23116-MS2-rep

    Plasmid

    #184842

    Purpose

    Expression of the rep protein of phage MS2

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Replicase gene MS2 phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23116

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-PERK(LD)-EGFP-HOTag3

    Plasmid

    #225678

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDD426

    Plasmid

    #202079

    Purpose

    Frame +1/-0 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CNR2-Tango

    Plasmid

    #66255

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CNR2 (CNR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PRK5-FLAG-Bag6ΔUBL

    Plasmid

    #61837

    Purpose

    express N-terminally FLAG tagged human Bag6 without UBL domain

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Use

    Tags

    Cre

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-VAMP7

    Plasmid

    #45913

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 7 (Vamp7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SIRT7 Flag

    Plasmid

    #13818

    Purpose

    Mammalian expression of human SIRT7 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT7 (SIRT7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Missing exon 2

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

23. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLQ14107 fwYellow-targeting sgRNA

    Plasmid

    #239456

    Purpose

    guide for fwYellow

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLQ3533 eforRed

    Plasmid

    #239451

    Purpose

    express eforRed chromoprotein

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    eforRed
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLQ3534 fwYellow

    Plasmid

    #239452

    Purpose

    express fwYellow chromoprotein

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLQ14112 tyrosinase-targeting sgRNA

    Plasmid

    #239751

    Purpose

    guide for tyrosinase

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLQ13960 tyrosinase(Priestia megaterium)

    Plasmid

    #239454

    Purpose

    express tyrosinase protein and melanin

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLQ14103 aeBlue-targeting_sgRNA

    Plasmid

    #239750

    Purpose

    guide for aeBlue

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting aeBlue
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCI-MEG3

    Plasmid

    #44727

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    Maternally Expressed Gene 3 (MEG3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge