161,273 results

1. pRecT_2

   Plasmid

   #167546

   Purpose

   Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Insert

   E. faecium Com15 recT under an IPTG inducible promoter, with lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   IPTG

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-ERBB2-V842I

   Plasmid

   #116359

   Purpose

   Lentiviral expression of ERBB2 V842I

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   V842I

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTK750

   Plasmid

   #114718

   Purpose

   Express mCherry-NuMA(5A-3) from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA 5A-3
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

   Plasmid

   #159130

   Purpose

   expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. N174-MCS (Puro)

   Plasmid

   #81068

   Purpose

   Lentiviral vector for constitutive gene expression.

   Depositor

   Adam Karpf

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMON-HsNMT1

   Plasmid

   #67475

   Purpose

   Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

   Depositor

   Richard Kahn

   Article

   Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

   Insert

   HsNMT1 (NMT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pBPGAL4.2Uw-2

   Plasmid

   #26227

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG-mCherry-sesRNA-GFP-WPRE-pA

   Plasmid

   #192065

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for Cheta
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1834

    Plasmid

    #142863

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EZH1 (EZH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC2SG5

    Plasmid

    #31283

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 May 27. ():.

    Insert

    Cry2 (Cry2 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

    Plasmid

    #63729

    Purpose

    To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Joseph Wu, Mark Kay

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Inserts

    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    SFFV

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p323-L10A-PATagRFP

    Plasmid

    #74172

    Purpose

    Fluorescent reporter for single ribosome tracking

    Depositor

    Robert Singer

    Article

    Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

    Insert

    ribosomal protein L10A (Rpl10a Rat)
    

    Use

    Lentiviral

    Tags

    PATagRFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-MVPH

    Plasmid

    #235600

    Purpose

    Expresses the MS2-VP64-P65-HSF1 fusion protein

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    MS2-VP64-P65-HSF1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Use

    Tags

    P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TEVSH

    Plasmid

    #125194

    Purpose

    His-tagged TEV protease with solubility increasing mutations

    Depositor

    Helena Berglund

    Article

    van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

    Insert

    TEV_SH
    

    Use

    Tags

    V5 His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-FRA1

    Plasmid

    #188665

    Purpose

    Expresses murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR hEF1A-BFP-P2A-rtTA-WPRE

    Plasmid

    #223288

    Purpose

    A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    BFP-P2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRDA_526

    Plasmid

    #184859

    Purpose

    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-PGK-hESRRB

    Plasmid

    #60434

    Purpose

    Over-expression of human ESRRB

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    ESRRB (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCW57-MCS1-P2A-MCS2 (RFP)

    Plasmid

    #80923

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

25. pmiHiFiCas9

    Plasmid

    #164832

    Purpose

    miHiFiCas9 expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    change R 691 to A

    Promoter

    CMV

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-EGFP-RhoA-wt

    Plasmid

    #12965

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid

    #197028

    Purpose

    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D52R / T132R

    Promoter

    MHCK7, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mIFP-TOMM20-N-10

    Plasmid

    #56239

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    NM_014765.2 (TOMM20 Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pMC-6-Ruby-9

    Plasmid

    #197740

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Ruby reporter tricistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge