165,688 results

1. pmCherry-NLS

   Plasmid

   #39319

   Depositor

   Martin Offterdinger

   Article

   Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

   Insert

   mCherry-3xNLS
   

   Tags

   3xSV40LT NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-RGS18HIS-kanMX6

   Plasmid

   #26882

   Depositor

   Peter Kaiser

   Article

   Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

   Insert

   18xHis tag
   

   Use

   Pcr tagging yeast

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28sumo-hRig-I-Full-length

   Plasmid

   #210827

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

   Insert

   hRIG-I (DDX58) Full length protein (RIGI Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

   Plasmid

   #226577

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   I178A, L182A, L186A, V190A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

   Plasmid

   #226578

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-AXL

   Plasmid

   #116714

   Purpose

   Lentiviral expression of AXL

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSV-TK

   Plasmid

   #165801

   Purpose

   Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   HSV-TK
   

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    pBAD/tetO

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-gw

    Plasmid

    #37626

    Purpose

    Non-integrating (episomal) expression vector with Gateway cassette

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-HA-AR-NTD

    Plasmid

    #171235

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-TRP1-PGAL1

    Plasmid

    #41606

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    TRP1 reverse orientation

    Available Since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-m-Paxillin

    Plasmid

    #80023

    Purpose

    Mammalian expression of mouse EGFP-Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_TGFBR1_WT

    Plasmid

    #82237

    Purpose

    Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TGFBR1 (TGFB1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUD-MSP1D1

    Plasmid

    #161608

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans and tyrosines w…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. anxA1-GFP

    Plasmid

    #107194

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A1
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. FUW-TetO-lox-ERAS

    Plasmid

    #52417

    Purpose

    Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. UbcH5A

    Plasmid

    #61081

    Purpose

    express a human E2

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    UbcH5A (UBE2D1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pBS-85xtetO-KlURA3

    Plasmid

    #44757

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    85x tetO array + KlURA3 marker + CHRI1 homology
    

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBR322-TIMER

    Plasmid

    #103056

    Purpose

    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMRXIP GFP-Stx18

    Plasmid

    #45918

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    syntaxin 18 (STX18 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pLVX-RNaseHI-NES-EGFP

    Plasmid

    #196701

    Purpose

    Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    bacterial RNaseHI
    

    Use

    Lentiviral

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBAD18HMGR

    Plasmid

    #19041

    Depositor

    Jay Keasling

    Article

    Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

    Insert

    truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only