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Showing: 11611 - 11640 of 158095 results

  2. MRGPRX2-Tango

    Plasmid
    #66440
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX2 (MRGPRX2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pET-28a(+)::NL

    Plasmid
    #141289
    Purpose
    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141289-plasmid-map-sequence-id-324904

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  17. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  20. pSHDY

    Plasmid
    #137661
    Purpose
    Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.
    Depositor
    Ilka Axmann
    Article
    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137661-plasmid-map-sequence-id-271675

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  23. pHS3

    Plasmid
    #81038
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    NATMX - HO endonuclease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pIRES LifeAct-GFP puro3

    Plasmid
    #64357
    Purpose
    Bicistronic expression of LifeAct-GFP fusion and puro3 resistance gene
    Depositor
    Matthieu Piel
    Insert
    LifeAct-GFP
    Use
    Tags
    IRES-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Lesley Forrester, Antonella Fidanza
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  30. pLEW100v5-177-HYG

    Plasmid
    #24013
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    K520R
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24013-plasmid-map-sequence-id-392337

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Showing: 11611 - 11640 of 158095 results