We narrowed to 19,203 results for: IRE
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Plasmid#121170PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.DepositorInsertABEmax-P2A-EGFP-ires-puro
UseCRISPRTagsP2A-EGFP-ires-puroExpressionMammalianAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2 Y637S-IRES-GFP
Plasmid#91887PurposeRetroviral expression of mouse Ezh2 with Y637SDepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationY637SPromoterMMLV gagAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo
Plasmid#62345PurposeExpresses MCP-tdiRFP670 in mammalian cellsDepositorInsertMCP-tdiRFP670
TagsHA and SV40 NLSExpressionMammalianPromoterCAG promoterAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
ExpressionMammalianMutationT27stopAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG
Plasmid#81022PurposeCancer-associated ASXL1 truncation mutant in retroviral vectorDepositorInsertASXL1 (ASXL1 Human)
Tags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoter5’LTRAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA
Plasmid#178518PurposeFor making Rabies virus SAD B19 strain L cell line with hyPBaseDepositorInsertB19L-IRES-mCherry
ExpressionMammalianAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2
Plasmid#113890PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1859A
Plasmid#169821PurposeExpresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationS1859A; TCCC -> AGTC silent mutations at nt527…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
Plasmid#153071PurposeLentiviral vector expressing human ZC3H12D mutated (D95N)DepositorInsertMutated ZC3H12D (D95N) (ZC3H12D Human)
UseLentiviralExpressionMammalianMutationChanged D95 to N, known to abrogate the RNase enz…PromoterEF1αAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry
Plasmid#136603PurposeEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertFKBP-C-mSOG-J-alpha
ExpressionMammalianPromoterCMVAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin
Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP
Plasmid#122954PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expressionDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-NGN2-IRES-GFP-T2A-Sox11
Plasmid#90213PurposeTo convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-Gluc-RMA-IRES-EGFP
Plasmid#189628PurposeExpresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and TREAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65
Plasmid#32569DepositorInsertsEGFP
Gal4p65
UseLentiviralPromoter2TPHAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
AiP935-Batf3-IRES2-FlpO-WPRE-neo
Plasmid#181771Purposetargeting vectorDepositorInsertBatf3 homology arms, FlpO
ExpressionMammalianAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianPromoterEFS and IRESAvailable SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204343PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3/Gbetagamma ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSFI-R-L301S-IRES-GFP
Plasmid#133744Purposeretroviral expression of CSF1-R mutantDepositorAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …Promotereno2 from zebrafishAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
Plasmid#122991PurposeExpresses Flag-tagged hRPGRIP1L in mammalian cellsDepositorAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR
Plasmid#200636PurposeStrong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertEGFP
ExpressionMammalianAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204342PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-s/Gbetagamma ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX-uORF-HA-birA*-STOP-IRES-Puro
Plasmid#120558PurposeExpresses control HA-birA* in mammalian cells & for virus productionDepositorInsertbirA
UseLentiviralTagsHAExpressionMammalianMutationR118GPromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF2-V2-FLAG-IRES-GFP
Plasmid#69049Purposelentiviral expression of IKZF2 variant 2 with FLAG tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationsplice variant 2PromoterCAGAvailable SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pGK-nAChRb2(E61C)-IRES-GFP
Plasmid#164777Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseLentiviralExpressionMammalianMutationE61CPromoterpGKAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP
Plasmid#18811DepositorInsertChx10 (164bp crit)+SV40p-tdTomato-IRES-AP
ExpressionMammalianAvailable SinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-TLR4 delta tail
Plasmid#133063PurposeRetroviral expression of mouse TLR4 with out its cytosolic tailDepositorAvailable SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST Halo-flag-CAD
Plasmid#188117PurposeExpresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tag and Halo tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-CD4-TLR4 cyto
Plasmid#133064PurposeRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4DepositorAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry
Plasmid#75132PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(E164K)
Plasmid#198569PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K107/113R-3xFlag_IRES-GFP
Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS
Plasmid#131355PurposeExpresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimusDepositorInsertCaspase-1 (C285A)-CpLxIS (Casp1 Mouse)
UseRetroviralExpressionMammalianMutationCaspase-1 (C285A)Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA
Plasmid#204547PurposeExpresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cellsDepositorAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP
Plasmid#75118PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomainDepositorAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
TagsFlagExpressionMammalianPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable SinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP
Plasmid#75129PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-016 FLAG UnaG[1] IRES UnaG[2]
Plasmid#130126PurposePPI (BiFC/TriFC) assay vectorDepositorInsertUnaG
ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only