162,717 results

1. pBbS5a-Opto-Cre-Vvd-2

   Plasmid

   #160400

   Purpose

   Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd-2
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NP-11-64-1

   Plasmid

   #234359

   Purpose

   Expressing rubisco and prk in Δrpi

   Depositor

   David Savage

   Article

   Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   R.rubrum rubisco
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-mRuby4(1-10)

   Plasmid

   #165143

   Purpose

   Expresses mRuby4(1-10) in mammalian cells

   Depositor

   Daichi Kamiyama

   Article

   Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

   Insert

   mRuby4(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eMagB-TgRFP-T

   Plasmid

   #162252

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagB-TgRFP-T
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PPDK

   Plasmid

   #64037

   Purpose

   Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

   Depositor

   Vern Schramm

   Article

   Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

   Insert

   pyruvate phosphate dikinase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBbS5a-OptoCre-REDMAP

   Plasmid

   #228465

   Purpose

   Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

   Depositor

   Mary Dunlop

   Article

   Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

   Insert

   OptoCre-REDMAP
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacUV5

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-EF1afull-H2B-mCherry-P2A-Fluc

   Plasmid

   #207253

   Purpose

   Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   H2B-mCherry-P2A-Fluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_full

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGOAL19

   Plasmid

   #20190

   Depositor

   Tanya Parish

   Article

   Parish et al Microbiology (Reading). 2000 Aug;146 ( P

   Type

   Empty backbone

   Use

   Marker cassette

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEB3-mScarlet3_N1

    Plasmid

    #189770

    Purpose

    Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    EB3-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eMagA-EGFP-Mito

    Plasmid

    #162244

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagA-EGFP-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNC-Cam1304-RNAi

    Plasmid

    #193399

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSm-Nuclease

    Plasmid

    #132431

    Purpose

    Expresses Serratia marcescens nuclease in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    Serratia marcescens nuclease (codon optimized)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGW-GFP-TurboID-GOI

    Plasmid

    #209381

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-myc3-bTrCP

    Plasmid

    #20718

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    beta-transducin repeat containing (BTRC) (Btrc Mouse)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3.1-Myoferlin HA

    Plasmid

    #22443

    Depositor

    William Sessa

    Article

    Bernatchez et al J Biol Chem. 2007 Oct 19. 282(42):30745-

    Insert

    Myoferlin
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Mito-RA

    Plasmid

    #209866

    Purpose

    Dimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR_SFFV_H2B_miRFP703

    Plasmid

    #231951

    Purpose

    Lentiviral vector for constitutive expression of the far-red fluorescent protein miRFP703 fused with the H2B nuclear localization tag (SFFV promoter)

    Depositor

    Leonardo Morsut

    Insert

    H2B_miRFP703
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3 Flag Pu.1

    Plasmid

    #66974

    Purpose

    Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Spi1 (Spi1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

23. eMagAF-EGFP-PM

    Plasmid

    #162247

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA3 Flag Jnk3a1

    Plasmid

    #13758

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a1 (MAPK10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pLenti-CMV-GFP-Sox11

    Plasmid

    #120387

    Purpose

    over expression of sox11 using infection

    Depositor

    Igor Ulitsky

    Article

    Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

    Insert

    sox11 (Sox11 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge