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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Publication

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Publication

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SMAD3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE1

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Publication

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Publication

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-parkin WT

Plasmid

#45875

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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mCh-Rab5

Plasmid

#49201

Purpose

expression of Rab5 fused to mCherry

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5 (RAB5B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pscAAV-GFP

Plasmid

#32396

Depositor

John T Gray

Publication

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pRS300

Plasmid

#22846

Purpose

Backbone for expressing plant artificial miRNAs

Depositor

Detlef Weigel

Publication

Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

Insert

MIR319a (MIR319a Mustard Weed)

Use

RNAi; Plant gene inactivation

Expression

Plant

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pEcCas

Plasmid

#73227

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

Depositor

Sheng Yang

Publication

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Inserts

Cas9
sgRNA

Use

CRISPR; Red recombinase

Expression

Bacterial

Promoter

native promoter

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pUC57-mitoLbNOX

Plasmid

#74448

Purpose

This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mitoLbNOX

Tags

Flag tag with linker and mitochondrial targeting …

Mutation

Human codon optimized

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMK289 (mAID-mClover-NeoR)

Plasmid

#72827

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mAID-mClover-Neo

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pet30b-7M SrtA

Plasmid

#51141

Purpose

expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Insert

S.aureus SrtA 7M

Tags

6x His

Expression

Bacterial

Mutation

P94R, E105K, E108Q, D160N, D165A, K190E, K196T

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Publication

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5X HRE of VEGF (VEGFA Human)

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pCAGGS-flpE-puro

Plasmid

#20733

Purpose

Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

FLPe recombinase

Use

Flp/frt

Expression

Mammalian

Promoter

CAGGS

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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