We narrowed to 7,692 results for: Lif;
-
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVMutationT230I (please see depositor comments below)PromoterTREAvailable SinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS2
Plasmid#128106PurposeExpress HA tagged Nrxn1α-LNS2DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 GFP-Atl1
Plasmid#86679PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS4
Plasmid#128108PurposeExpress HA tagged Nrxn1α-LNS4DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS5
Plasmid#128109PurposeExpress HA tagged Nrxn1α-LNS5DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-Map4
Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBD-Fc
Plasmid#164221PurposeExpresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporterDepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-Giantin
Plasmid#186575PurposeExpression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)DepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1b.WPRE.SV40
Plasmid#100857PurposeAAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1InsertjRGECO1b
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:GtACR2-tdTomato
Plasmid#124236PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomatoDepositorInsertGtACR2-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA
Plasmid#84467PurposeEmpty pAAV-CRAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBMN-mCherry-Parkin
Plasmid#59419Purposeto make mCherry-Parkin stable cell line with retrovirusDepositorAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorAvailable SinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDM1383
Plasmid#207423PurposeExtrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)DepositorAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔRING-Box
Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
TagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
TagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-YFP-NLS-STOP-L2
Plasmid#127362Purposegateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ heiCas9
Plasmid#186929PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLSDepositorInsertmyc-flexible linker-oNLS-Cas9-oNLS
UseCRISPRTagsmyc-flexible linker-optimizedNLS and optimizedNLSExpressionMammalianPromotersp6Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLacZ-1735
Plasmid#115454PurposeConstitutive lentiviral expressionDepositorInsertshLacZ-1735
UseLentiviral and RNAiExpressionMammalianAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP
Plasmid#139283PurposeExpress hChR2(H134R)-P2A-EYFP in a Cre-dependent mannerDepositorInserthChR2(H134R)-P2A-EYFP
UseAAVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Pansio
Plasmid#214441PurposeBxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2A
Plasmid#115330Purposeexpresses mCherry-CHMP2A in mammalian cellsDepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-FRB
Plasmid#186576PurposeExpression of mCherry-FRB at the outer surface of the lysosome using LAMP1DepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYP-GFP
Plasmid#197283PurposeEncodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.DepositorInsertSYP and GFP (Syp Rat)
UseLentiviralTags1D4 epitopeMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2B
Plasmid#115331Purposeexpresses mCherry-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationsilent mutation in position 4205 C to TPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CKAR2
Plasmid#123348PurposeImproved FRET-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCKAR2
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-YFP-NLS-STOP-L2
Plasmid#127361Purposegateway entry vector for expressing nuclear TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ntn4-Fc-His
Plasmid#72106PurposeExpresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p8325 pLIX YAP1 WT
Plasmid#184527PurposeExpression of YAP1 WTDepositorAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-CHMP2A
Plasmid#115328Purposeexpresses mEGFP-CHMP2A in mammalian cellsDepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.z-AP-His
Plasmid#72053PurposeExpresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)
Plasmid#193048PurposeHeatshock expressed Cas9DepositorInserthsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
UseCRISPRExpressionWormPromoterhsp16.41pAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 V5 mDispatched WT 2x HA
Plasmid#126410PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.DepositorInsertm-Dispatched1 (Disp1 Mouse)
TagsHAExpressionMammalianMutationV5 iserted between A106 and A107Available SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-YFP
Plasmid#108853PurposeEncodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROR2-V5/HIS
Plasmid#205608PurposeExpression of human ROR2 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-3xrGBD
Plasmid#176108PurposeVisualization of active RhoDepositorAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-NES-L2
Plasmid#127349Purposegateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS8
Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Plxna4-Fc-His
Plasmid#72125PurposeExpresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-Halo
Plasmid#195163Purposemammalian expression of siRNA-resistant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
TagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-AP-His
Plasmid#71978PurposeExpresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCB619
Plasmid#171011PurposeCRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).DepositorInsertU6:sgRNA_Puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only