We narrowed to 34,416 results for: CaS
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Plasmid#138317PurposeLenti expression of SpyCas9 with Puro selectionDepositorInsertSpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptreCas9-mKate2ps-T1gRNA
Plasmid#62715PurposeInducible; gRNA seq is ATGAGAATCAAGGCGGTCGADepositorInsertCas9–mKate2ps
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 L-FKBP-Cas9
Plasmid#162728PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (insertion 5)
Plasmid#128116PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)DepositorInsertCascAID (insertion 5)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (N39A)
Plasmid#128117PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (N39A)DepositorInsertCascAID (N39A)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:EDLL:tNos (GB1190)
Plasmid#75404PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatorDepositorInsertdCas:EDLL
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-HMGB1
Plasmid#183198PurposeEntry cloneDepositorInsertCas9-HMGB1
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PrCas12a-2AeGFP
Plasmid#123636PurposeMammalian expression, Genome editingDepositorInsertPrCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, A
Plasmid#107795PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, B
Plasmid#107796PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC4
Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
RCAS (E) shh (CT#489)
Plasmid#13992DepositorInsertshh (SHH Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) mPax1 (CT#1039)
Plasmid#13902DepositorInsertPax1 (Pax1 Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-Casp2-shRNA
Plasmid#157926PurposeKnockdown of caspase-2DepositorInsertCasp2 shRNA (Casp2 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMutationPromotermouse U6Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
RCAS IkB alpha M
Plasmid#12407DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas6
Plasmid#106274PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasE
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) mWnt1 (CT#165)
Plasmid#14017DepositorInsertWnt1 (Wnt1 Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas5c/pHMGWA
Plasmid#81182PurposeExpresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas5c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas8c/pHMGWA
Plasmid#81183PurposeExpresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas8c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCJH031_proD_LbCas12a-gRNA-GFP_CAM
Plasmid#183075PurposePlasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertLbCas12a guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) cWnt5b (CT#851)
Plasmid#13975DepositorInsertWnt5b (WNT5B Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMS25: pTns(PmcCAST)
Plasmid#168158PurposeInducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-eSpCas9
Plasmid#133370PurposePiggybac vector for constitutive expressionDepositorInserteSpCas9
UseTagsFlagExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3
Plasmid#107728PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 dCas9 (GB2664)
Plasmid#160596PurposeCDS of dCas9 with B3 nomenclatureDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(B) cFgf8 (CT#807)
Plasmid#13892DepositorInsertFgf8 (FGF8 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-eSpCas9-sgRNA
Plasmid#179580PurposeExpresses eSpCas9 and gRNA that targets ColE1 plasmids for curingDepositorInserteSpCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas8e
Plasmid#106270PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasA
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas7
Plasmid#106272PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasC
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas5
Plasmid#106273PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasD
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPcCas12a
Plasmid#174681PurposeExpresses 6xHis-MBP tagged PcCas12aDepositorArticleInserthuPcCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huCMtCas12a
Plasmid#174680PurposeExpresses 6xHis-MBP tagged CMtCas12aDepositorArticleInserthuCMtCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) cNodal (CT#343)
Plasmid#14014DepositorInsertNodal (NODAL Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPdCas12a
Plasmid#174682PurposeExpresses 6xHis-MBP tagged PdCas12aDepositorArticleInserthuPdCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYC-OspCas12c-noncoding
Plasmid#120878PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-OspCas12
UseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA-1050S (UASt-dCasRx)
Plasmid#132419PurposeExpress dCasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
UseTagsExpressionInsectMutationPromoterUAStAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVMG0173_Cq-Actin5c-Cas9
Plasmid#169345PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0173_Cq-Actin5c-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009808Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) cTbx5 (CT#634)
Plasmid#13963DepositorInsertTbx5 (TBX5 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) cScx HA (CT#744)
Plasmid#13889DepositorInsertScleraxis (SCX Chicken)
UseRetroviral; Avian expressionTagsHAExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAC148-pCR8-dCas9VP96
Plasmid#48220PurposedCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP96ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0212_Cq-nanos-Cas9
Plasmid#169348PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0212_Cq-nanos-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ011551Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0213_Cq-vasa-Cas9
Plasmid#169347PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0213_Cq-vasa-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009286Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (wtAcrIIA4)
Plasmid#128115PurposeExpresses SpyCas9 fused to wild-type AcrIIA4DepositorInsertCascAID (wtAcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only