We narrowed to 14,145 results for: TIM

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pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ERoxBFP

Plasmid

#68126

Purpose

mammalian expression of ER localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

oxBFP

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-2

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_3xHA

Plasmid

#112097

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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OeNL/pcDNA3

Plasmid

#85202

Purpose

Orange color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Orange enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pASTA3

Plasmid

#24657

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

tdTomato

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pPL5606_TEF1*-Cre_ADE2

Plasmid

#60932

Purpose

Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pOz-humBirA-GFP

Plasmid

#133283

Purpose

Expression of BirA-GFP fusions in mammalian cells

Depositor

Arman Kulyyassov

Article

Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

Insert

eGFP

Use

Retroviral

Tags

Humanized wild type biotin ligase BirA

Expression

Mammalian

Promoter

MoMuLV

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-HA-mTmod3-WT

Plasmid

#70752

Purpose

N-terminal-HA-tagged mouse Tropomodulin3, WT

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-SpHis5

Plasmid

#44836

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-cdaR

Plasmid

#62557

Purpose

Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Inserts

beta lactamase
sfGFP
CdaR

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoLSS-mKate2-SpHis5

Plasmid

#44848

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-CyRFP1

Plasmid

#84356

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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ReNL_pRSETb

Plasmid

#89536

Purpose

Red color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-SpHis5

Plasmid

#44841

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-ATP

Plasmid

#184599

Purpose

For expression of a fluorescent sensor for ATP in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ATP

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL5608_TEF1*-Cre_TRP1

Plasmid

#60931

Purpose

Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

Mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pH_35S/mEGFP

Plasmid

#135321

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
[pSK600] pRK5 HA-Nprl2(R78A)

Plasmid

#136146

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

Insert

Nprl2 (NPRL2 Human)

Tags

HA

Expression

Mammalian

Mutation

R78A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-BICD2(1-500)-FRB

Plasmid

#174648

Purpose

Rapalog-inducible coupling to dynein adaptor BICD2

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

HA-BICD2(1-500)-FRB

Tags

FRB and HA

Expression

Mammalian

Mutation

mmBICD2(aa1-500): Lys213Glu

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS019: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163048

Purpose

ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-Cas9

Plasmid

#64237

Purpose

expresses codon optimized Cas9 in zebrafish

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

codon optimized Cas9

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_NES_HaloTag9

Plasmid

#175537

Purpose

Strict cytosolic expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA0718_pPB-TREtight-cHA-IRESVenus

Plasmid

#124171

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRsetB-RoTq-Off

Plasmid

#234466

Purpose

Bacterial expression vector for RoTq-Off

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-Off

Tags

7xHis

Expression

Bacterial

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-NKB

Plasmid

#184609

Purpose

For expression of a fluorescent sensor for neurokinin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NKB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_33_pTEFmut8-mCherry

Plasmid

#177706

Purpose

Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEFmut8-mCherry

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CIp10 - mTurquoise2 (γmGFP based)

Plasmid

#163121

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mTurquoise2 - γmGFP based

Use

Candida albicans expression

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.683

Plasmid

#108531

Purpose

BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

BetIAM + PBetI-YFP

Expression

Bacterial

Mutation

BetI: S115I

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUNIV-EGFP

Plasmid

#24706

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Insert

EGFP (eGFP Aequoria victoria)

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoTagRFP-T-CaURA3

Plasmid

#44877

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB_PalkB_sfgfp_KanR

Plasmid

#166503

Purpose

Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PalkB

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_mNeonGreen

Plasmid

#191743

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mNeonGreen

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 14,145 results for: TIM