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Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
UseTags6XHisExpressionYeastMutationPlease see depositor commentsPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-CaURA3
Plasmid#44881PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP
Plasmid#175522PurposeNuclear expression of HaloTag11 in mammalian cellsDepositorInsertH2B-HaloTag11
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA2058_pX330A-G2P
Plasmid#124202PurposeExpression of SpCas9 and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Venus SX
Plasmid#103990PurposePlasmid encoding Venus SXDepositorInsertVenus SX
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-CCK
Plasmid#184602PurposeFor expression of a fluorescent sensor for CCK in mammalian cellsDepositorInsertMTRIA-CCK
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-170nDelta13
Plasmid#13562DepositorInsertFLIPglu F16F Delta13
UseTagsECFP and EYFPExpressionBacterialMutationWT binding protein (no F16A)PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Crimson/P2A-ICP8 (pSLIK1)
Plasmid#113856PurposeFor Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8DepositorInsertICP8
UseLentiviralTagsP2A/CrimsonExpressionMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCJJ344
Plasmid#117503PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtUBI10Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-SpHis5
Plasmid#44857PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selectionDepositorInsertyoClover
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
KA1390_pGL4-UBCmin-luc2
Plasmid#124185PurposeLuciferase reporter with the UBC minimal promoterDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL50016
Plasmid#50315DepositorInsertCDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
UsePlant expressionTagsExpressionMutationCodon-optimised for A. thaliana. BsaI/BbsI sites …PromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-3.2mDelta13
Plasmid#13560DepositorInsertFLIPglu F16A S112A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A, S112A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mApple
Plasmid#132566PurposemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmApple
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-SpHis5
Plasmid#44850PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selectionDepositorInsertyomWasabi
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII275Pglu-4.6m
Plasmid#13565DepositorInsertFLIPglu F16A G275S-ECFP-K276R
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEBB-XIAP1-351
Plasmid#11560DepositorInsertXIAP1-351 (XIAP Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 352-496PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET17
Plasmid#107443PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET17 (MET17 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185H/C280S in pET15b
Plasmid#61672PurposeHIV-1 integrase F185H/C280S in pET-15bDepositorInsertHIV-1 integrase F185H/C280S
UseSynthetic BiologyTagsHisExpressionMutationPromoterT7 promotorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-MmTFP13xmyc
Plasmid#86371Purposemembrane targeted Tfp1 expression in zebrafishDepositorInsertmTfp1
UseZebrafishTags3xMyc and GAP43 membrane targetingExpressionMutationPromoterUASAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-SpHis5
Plasmid#44856PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-lifeact-mAPPLE
Plasmid#89820PurposemAPPLE labeled fluorescent actinDepositorInsertlifeact-mAPPLE
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mScarlet
Plasmid#191745PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mScarletExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-2uDelta13
Plasmid#13561DepositorInsertFLIPglu F16F D152A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A, D152A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
YeNL-H2B_pcDNA3
Plasmid#89522PurposeYellow color variant of bright luminescent protein, fused to histone 2BDepositorInsertYeNL
UseTagshistone 2BExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChEF-citrine
Plasmid#50975PurposeAAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITRDepositorInsertChEF-citrine
UseAAVTagscitrineExpressionMutationChEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
CyclinB-GFP
Plasmid#54465PurposeCyclinB-GFP in pIVT vector for in vitro transcriptionDepositorInsertCyclinB
UseTagsGFPExpressionMammalianMutationK67E and I150T mutations compared to GenBank refe…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-mScarlet (MNW006)
Plasmid#174136PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-dH3-YFP
Plasmid#8694DepositorInsertH3 (His3:CG31613 Fly)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet17
Plasmid#107453PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet17 (met17 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mApple
Plasmid#191741PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mAppleExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mCherry
Plasmid#191742PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mCherryExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS031: superecliptic pHluorin (endoplasmic reticulum)
Plasmid#163051Purposesuperecliptic pHluorin (endoplasmic reticulum)DepositorInsertsuperecliptic pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
tubulin-CeNL_pcDNA3
Plasmid#89515PurposeCyan color variant of bright luminescent protein, fused to b-tubulinDepositorInsertCeNL
UseTagsb-tubulinExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-SpHis5
Plasmid#44848PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selectionDepositorInsertyoLSS-mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-7SK-Blast
Plasmid#183620PurposelentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterModified 7SKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHT-trxA.ads
Plasmid#47383PurposeExpresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHis and trxAExpressionBacterialMutationPromoterPgracAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtUBI10Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-PRLH
Plasmid#184618PurposeFor expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cellsDepositorInsertMTRIA-PRLH
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-NOC
Plasmid#184611PurposeFor expression of a fluorescent sensor for nociceptin in mammalian cellsDepositorInsertMTRIA-NOC
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only