174,341 results
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Plasmid#75125PurposeRetroviral expression plasmid encoding wild type human EZH2DepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA-gp78-FLAG
Plasmid#62370PurposeExpress C-terminally FLAG tagged gp78DepositorAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
ExpressionBacterialPromoterownAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorInsertalpha-catenin FRET Sensor
TagsECFP and YPETExpressionMammalianPromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP1,3 #2515
Plasmid#74237PurposeModified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47DepositorInsertsAAV2 Rep
AAV9.47 VP1
AAV9.47 VP3
UseAAVAvailable SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
Plasmid#74289PurposepAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertH2B-GFP and oG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRExpressionBacterialPromoterpBADAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBV-Luc
Plasmid#71414PurposeTo see the activity of HBV transcription using Luciferase assayDepositorInsertHBV core promoter, enhancer I, enhancer II and luciferase
UseLuciferaseExpressionMammalianPromoterHBVAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Notch1 intracellular domain-pcw107-V5
Plasmid#64622Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestattB (pCM268)
Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVExpressionMammalianPromoterEF1aAvailable SinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ii-OVA.I+II
Plasmid#64596PurposeExpression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentationDepositorInsertOvalbumin
TagsInvariant chainExpressionMammalianMutationOnly amino acids 242-353 (class I and class II ep…PromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only