We narrowed to 17,375 results for: puro

1. METTL3 shRNA 2 tet pLKO puro

   Plasmid

   #162984

   Purpose

   Tet-inducible shRNA targeting human METTL3 #2

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP shRNA tet pLKO puro

   Plasmid

   #163017

   Purpose

   Control tet-inducible shRNA targeting eGFP

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPaint-APEX2-GFP-3xFLAG-PuroR

   Plasmid

   #221238

   Purpose

   CRISPaint gene tagging

   Depositor

   Jonathan Kagan

   Article

   Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

   Insert

   APEX2-GFP-3xFLAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

   Plasmid

   #154987

   Purpose

   Lentiviral vector expressing Human ACE2 and TMPRSS2

   Depositor

   Sonja Best

   Insert

   ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-EF1a-CCO-IRES-Puromycin

   Plasmid

   #134861

   Purpose

   Expresses EGFP-tagged inner mitochondrial membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome c oxidase subunit VIII (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSIN4-EF1a-ETV2-IRES-Puro

   Plasmid

   #61061

   Purpose

   lentiviral vector for ETV2

   Depositor

   Igor Slukvin

   Article

   Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti CMVTRE3G eGFP Puro (w819-1)

   Plasmid

   #27570

   Depositor

   Eric Campeau

   Insert

   Enhance Green Fluorescent Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-FLAG-puro-PTPIP51(1-470)

   Plasmid

   #170536

   Purpose

   Expresses PTPIP51(1-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CMV/TO Puro empty (w175-1)

    Plasmid

    #17482

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-Ef1a-hMGT-PGK-puro

    Plasmid

    #172394

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti CMV/TO eGFP Puro (w159-1)

    Plasmid

    #17481

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

    Plasmid

    #175037

    Purpose

    Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Sp sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TLCV2-Scal-T2A-EGFP-Puro

    Plasmid

    #234949

    Purpose

    Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TLCV2-ApaLI-T2A-EGFP-Puro

    Plasmid

    #234948

    Purpose

    Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe-Puro-Myr-Flag-AKT1

    Plasmid

    #15294

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSK-mU6-Cj-sgRNA-SV40-puro

    Plasmid

    #192128

    Purpose

    Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Cj-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV PIG (Puro IRES GFP)

    Plasmid

    #18751

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-EFIa-Id1-PGK-puro

    Plasmid

    #107735

    Purpose

    Expresses Id1 in mammalian cells

    Depositor

    Alexandre Colas

    Article

    Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

    Insert

    Id1 (T Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti CMV/TO RasV12 Puro (w119-1)

    Plasmid

    #22262

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Puro-T2A-mCitrine-hSmad2-donor

    Plasmid

    #203663

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    Smad2
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid

    #214125

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-TET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKIBra-P2A-mCherry-Puro-sfGFP

    Plasmid

    #115686

    Purpose

    Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

    Depositor

    David Suter

    Article

    Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

    Insert

    P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG Lenti CMV EGFP Puro

    Plasmid

    #236084

    Purpose

    Lentiviral backbone expressing EGFP, control for empty backbone 236083

    Depositor

    Melina Fan

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pHDR-FOXL2-T2A-tdTomato-PuroTK

    Plasmid

    #192892

    Purpose

    HDR donor for inserting FOXL2-T2A-tdTomato reporter

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2-tdTomato (FOXL2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    T2A-tdTomato fluorescent reporter

    Promoter

    PGK

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-EF1a-PTS1-IRES-Puromycin

    Plasmid

    #134665

    Purpose

    Expresses EGFP-tagged peroxisome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    tripeptide SKL (Peroxisome targeting signal 1)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid

    #72299

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLV-puro-N-3HA-SREBP2-C-Flag

    Plasmid

    #134287

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid

    #155307

    Purpose

    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV-MPAct-mRuby3-IRES-Puro

    Plasmid

    #155231

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mRuby sensor
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CMV-SauriCas9-KKH-Puro

    Plasmid

    #135966

    Purpose

    Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112927

    Purpose

    CRISPR-a reporter assay lentiviral vector (gTetO)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMJA285= pSin-TCRab-CMVp-Puro

    Plasmid

    #116875

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TCR (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion of EF1-a promotor

    Promoter

    CMV Promotor

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

    Plasmid

    #223160

    Purpose

    Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    dGFP-P2A-NanoLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBabe-TetCMV-puro-mCherry-PA-Rac1

    Plasmid

    #22035

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCherry-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Tet-CMV

    Available since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSIN4-EF1a-GATA1-IRES-Puro

    Plasmid

    #61062

    Purpose

    lentiviral vector for GATA1

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Human 3' HP1a AID GFP PuroR

    Plasmid

    #127906

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    HP1 (CBX5 Human, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

    Plasmid

    #165082

    Purpose

    gRNA 1 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid

    #227275

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pPB-CAG-Brainbow3.2-PGK-PuroTK

    Plasmid

    #159928

    Purpose

    Brainbow transgenesis

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

    Inserts

    NFPnls
    mOrange2
    EGFP
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV[TetOn]-Puro-TRE3G>mNeurog2

    Plasmid

    #198754

    Purpose

    Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Neurogenin-2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

    Plasmid

    #145839

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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