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Plasmid#106316PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy Flag GCN5
Plasmid#14106DepositorInsertGCN5 (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationE189G and K195NPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-eGFP
Plasmid#157734Purposemammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoterDepositorInsertFH-eGFP
UseTagsFlag-His8ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-TagRFP
Plasmid#162035PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsRFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-TTF-1-gRNA
Plasmid#101857PurposeIntroducing a gRNA targeting human TTF-1 (NKX2-1) into cellsDepositorInsertgRNA targeting TTF-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-sfGFP
Plasmid#116917PurposeStrong constitutive expression of super-folder GFP for cell-free expressionDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterJ23101Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1-Fc
Plasmid#164220PurposeExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporterDepositorInsertSpike S1 domain (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TMPRSS3
Plasmid#221298PurposeExpression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertTMPRSS3 (pLxIS) (Tmprss3 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA-WT (pFastBac)
Plasmid#16644DepositorInsertPIK3CA (PIK3CA Human)
UseBaculoviralTagsHisExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP-HF
Plasmid#157735Purposemammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP-HF
UseTagsHis8-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TASL(FL)
Plasmid#221263PurposeExpression of TASL in mammalian cells by retroviral transductionDepositorInsertTASL (Tasl Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVie-IRES-BlastR alt MCS
Plasmid#120862Purpose3rd generation lentiviral expression under a CMVie promoter with Blasticidin selectionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-UMPS-3xFLAG
Plasmid#87975Purpose3rd generation lentiviral vector for Expression of UMPS in mammalian cellsDepositorInsertUMPS (UMPS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NQO1
Plasmid#61730PurposeExpresses WT human NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-p3-PE(1152)-N-term
Plasmid#187181Purposep3-PE2-N-term(1152) in AAV2 backbone (ITRs)DepositorInsertPE2 from plasmid #132775
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-Luc
Plasmid#122694PurposeOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expressionDepositorInsertFirefly luciferase
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
Plasmid#139649PurposeReporter assayDepositorInsertH2B-mCherry
UseLentiviralTagsFLAG, His6ExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PIF6CreN
Plasmid#131780PurposeMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite systemDepositorInsertPIF6CreN
UseCre/Lox; Zebrafish expressionTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGMCtKq28-TSC10M1-sspB
Plasmid#49526PurposeKanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-TEAD-p-H2B-mCherry
Plasmid#139648PurposeReporter assayDepositorInsertIRES-GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-SopE
Plasmid#221327PurposeDox-inducible expression of SopE in mammalian cells by retroviral transductionDepositorInsertSopE
UseRetroviralTagsFLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1 GSK3beta S9A HA
Plasmid#15994DepositorInsertGSK3beta (GSK3B Human)
UseTagsHAExpressionMammalianMutationS9A: constitutively activePromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorInsertSmpd4 (Smpd4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-NDUFB2
Plasmid#139841PurposeLentiviral expression of human NDUFB2DepositorInsertNDUFB2 (NDUFB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP
Plasmid#162032PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FSW-ALDH2-E504K-T2A-EGFP
Plasmid#197037PurposeExpress ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFPDepositorInsertALDH2-E504K
UseLentiviralTagsExpressionMammalianMutationE504KPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT1
Plasmid#106304PurposeExpress SHMT1DepositorInsertserine hydroxymethyltransferase 1 (SHMT1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterPBenAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T38S38
Plasmid#49524PurposeAmpicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertconstitutive TetR38
UseTagsExpressionMutationPromoterPtb38Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P750
Plasmid#49525PurposeAmpicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertP750
UseTagsExpressionMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only