We narrowed to 6,273 results for: nls
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Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY120 (pcDNA3.1-huFbCpf1)
Plasmid#92296PurposeExpression of humanized FbCpf1DepositorInserthuFbCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY126 (pcDNA3.1-huBoCpf1)
Plasmid#92302PurposeExpression of humanized BoCpf1DepositorInserthuBoCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY123 (pcDNA3.1-huCMaCpf1)
Plasmid#92299PurposeExpression of humanized CMaCpf1DepositorInserthuCMaCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY114 (pcDNA3.1-huPgCpf1)
Plasmid#92273PurposeExpression of humanized PgCpf1DepositorInserthuPgCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY122 (pcDNA3.1-huCPbCpf1)
Plasmid#92298PurposeExpression of humanized CPbCpf1DepositorInserthuCPbCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSMutationE174R, S542R, K548RPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-BnTW
Plasmid#133365PurposePiggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLSDepositorInserttdTomato-NLS
TagsFlag-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available SinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
CROPSeq-PP7-p65-HSF
Plasmid#196719PurposeCROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nucleus-7
Plasmid#54206PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
TagsmEmerald-NucleusExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMG005_mNeonGreen_P2A
Plasmid#237215PurposeFluorescent reporter codon optimized for Dictyostelid speciesDepositorInsertmNeonGreen-P2A
UseCloningTags3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMG008_mCherry_P2A
Plasmid#237216PurposeFluorescent reporter codon optimized for Dictyostelid speciesDepositorInsertmCherry-P2A
UseCloningTags3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorInsertpEBFP2-Nuc
TagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCAS-pA
Plasmid#48625PurposeExpresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized Cas9
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianPromoterCAG promoterAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmKalama1-Nuc
Plasmid#14894PurposeMammalian expression of mKalama1 blue fluorescent protein with NLSDepositorInsertpmKalama1-Nuc
TagsNLSExpressionMammalianMutationI178TPromoterCMVAvailable SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
TC512
Plasmid#122615PurposePmig-13::Zip(-)::QFAD::NLS::SL2::mCherryDepositorInsertZip(-)::QFAD::NLS::SL2::mCherry
ExpressionWormAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB754
Plasmid#60157PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLYS9prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB691
Plasmid#60135PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertRdLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB753
Plasmid#60148PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLEU1prAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB689
Plasmid#60133PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB756
Plasmid#60149PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLEU1prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB752
Plasmid#60155PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterADE17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD443
Plasmid#168469PurposeFor the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mScarlet-I-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSP33
Plasmid#199375PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSP31
Plasmid#199373PurposeEncodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BII-gR-PnTW
Plasmid#133356PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLSDepositorInserttdTomato-NLS
TagsHA-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSP23
Plasmid#199372PurposeEncodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP
Plasmid#228359PurposeRfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdownDepositorInsertRfxCas13d
UseLentiviralTags2A-EGFP, 2A-mCD4, HA, and NLSExpressionMammalianPromoterEF1AAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TC513
Plasmid#122616PurposePunc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherryDepositorInsertSV40 NLS::rtetR::Zip(+)::SL2::mCherry
ExpressionWormAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-5
Plasmid#115626PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertNLS-nCas9-NLS-wtTadA-TadA7.10-NLS
UseCRISPRExpressionPlantAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only