We narrowed to 5,797 results for: plasmid dna

1. HySp5-227

   Plasmid

   #192996

   Purpose

   Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Sp5
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no SP box and DNA binding domain

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HySp5-337

   Plasmid

   #192994

   Purpose

   Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Sp5
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no DNA binding domain

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCL.103

   Plasmid

   #184988

   Purpose

   Express Cas9-P2A-Eco1RT

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.027

   Plasmid

   #184964

   Purpose

   Express -Eco1 RT and ncRNA in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCL.102

   Plasmid

   #184987

   Purpose

   Express -Eco1 RT-P2A-Cas9

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-P2A-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOD_003

   Plasmid

   #155362

   Purpose

   Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

   Plasmid

   #107300

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP
   

   Use

   CRISPR

   Tags

   3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. rs210-major

    Plasmid

    #113377

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. rs210-minor

    Plasmid

    #113378

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rs438-major

    Plasmid

    #113369

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs4384382 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

14. rs344-minor

    Plasmid

    #113366

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs34466261 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. rs438-minor

    Plasmid

    #113370

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs4384382 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. rs294-minor

    Plasmid

    #113374

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2941742 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. rs294-major

    Plasmid

    #113373

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2941742 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. rs281-minor

    Plasmid

    #113372

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs281038 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. rs281-major

    Plasmid

    #113371

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs281038 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rs117-major

    Plasmid

    #113367

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs11716984 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. rs117-minor

    Plasmid

    #113368

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs11716984 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. rs676-major

    Plasmid

    #113375

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs67668514 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs67668514 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. rs676-minor

    Plasmid

    #113376

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs67668514 minor allele (-)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs67668514 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. rs214-major

    Plasmid

    #113379

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 major allele (C)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. rs700-major

    Plasmid

    #113361

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7007731 major allele (C)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7007731 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. rs700-minor

    Plasmid

    #113362

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7007731 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7007731 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. rs344-major

    Plasmid

    #113365

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 major allele (AC)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs34466261 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. rs699-minor

    Plasmid

    #113358

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs6990531 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs6990531 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. rs769-minor

    Plasmid

    #113364

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7697634 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7697634 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. rs769-major

    Plasmid

    #113363

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7697634 major allele (C)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7697634 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. rs622-minor

    Plasmid

    #113360

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs62288111 minor allele (C)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs62288111 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. rs622-major

    Plasmid

    #113359

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs62288111 major allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs62288111 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. rs795-minor

    Plasmid

    #113356

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7957274 minor allele (C)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7957274 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. rs795-major

    Plasmid

    #113355

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs7957274 major allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs7957274 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. rs699-major

    Plasmid

    #113357

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs6990531 major allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs6990531 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AX44_pS.Donor.S1

    Plasmid

    #100289

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AP1298‐1

    Plasmid

    #87248

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1

    Plasmid

    #237139

    Purpose

    Express MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MLL1 (KMT2A Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. NanoBRET Assay Vector, NanoLuc-NRAS WT

    Plasmid

    #236875

    Purpose

    Express NanoLuc(R)-NRAS WT in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    NRAS (NRAS Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

    Plasmid

    #236863

    Purpose

    Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    NRAS (NRAS Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    Q61R

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

    Plasmid

    #236854

    Purpose

    Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    NRAS (NRAS Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGL3-U6-sgRNA-PGK-puromycin

    Plasmid

    #51133

    Purpose

    expresses sgRNA from the U6 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. Lenti-multi-Guide

    Plasmid

    #85401

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMax_GFP

    Plasmid

    #177825

    Purpose

    Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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