161,592 results

1. pGL4 [luc2P/Myc/Hygro] Vector

   Plasmid

   #236815

   Purpose

   Express Myc response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   Myc (MYC Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   minP

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. EBFP-C1

   Plasmid

   #54738

   Purpose

   Localization: C1 Cloning Vector, Excitation: 387, Emission: 450

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pLKO.1 neo

   Plasmid

   #13425

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

   Depositor

   Sheila Stewart

   Insert

   non-hairpin 18bp
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSEAP

   Plasmid

   #37326

   Depositor

   John Schiller

   Article

   Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

   Insert

   SEAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SMVP-Cas9C

   Plasmid

   #80931

   Purpose

   Expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV3flag8HOIL-1L

   Plasmid

   #50016

   Purpose

   Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-DDX3X

   Plasmid

   #116730

   Purpose

   Lentiviral expression of DDX3X

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ23101

    Plasmid

    #70252

    Purpose

    Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

    Depositor

    Jim Haseloff

    Article

    Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

    Insert

    promoter J23101-RBS B0034-EYFP-B0015
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-CBE6d

    Plasmid

    #215822

    Purpose

    Express CBE6d (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GST-TEV-FUS

    Plasmid

    #29629

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    Sophie Helaine, David Holden

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Flag mTOR wt

    Plasmid

    #26603

    Purpose

    Mammalian expression of human flag-tagged mTor

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-Puro-miniAAVR-FLAG

    Plasmid

    #166717

    Purpose

    Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    miniAAVR-FLAG
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Only expressing PKD1-3 of ectodomain

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hSyn1_nLightG

    Plasmid

    #187179

    Purpose

    Expresses green norepinephrine indicator nLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

    Plasmid

    #199248

    Purpose

    Fluorescent labelling of bacterial cells with sfGFP

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized sfGFP gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC18T-mini-Tn7T-hph-Ptet1

    Plasmid

    #218367

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLminP_Luc2P_RE55

    Plasmid

    #90400

    Purpose

    C-REL - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CEBPa

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-IDH2-WT

    Plasmid

    #87926

    Purpose

    Expresses IDH2 (wild-type) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

    Plasmid

    #141372

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-HA-mUbp43

    Plasmid

    #12454

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    UBP43 (Usp18 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMRX-INU-FLAG-CCPG1

    Plasmid

    #128262

    Purpose

    Expresses CCPG1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    CCPG1 (CCPG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCerulean3-C1

    Plasmid

    #54605

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. tetO-TBX20

    Plasmid

    #170705

    Purpose

    doxycycline-inducible overexpression of TBX20

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TBX20 (TBX20 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge