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170,794 results

Showing: 2281 - 2320 of 170794 results

  2. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  5. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

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  13. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189755-plasmid-map-sequence-id-385774

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  18. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92120-plasmid-map-sequence-id-197614

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  19. SpRY-ABE8e(V106W)

    Plasmid
    #198553
    Purpose
    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198553-plasmid-map-sequence-id-388905

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  34. pl-Synapsin-YFP-NaVII-III

    Plasmid
    #91246
    Purpose
    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
    Depositor
    Matthew Grubb
    Article
    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.
    Insert
    Nav1.2 (Scn2a Rat)
    Use
    Lentiviral
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    The insert codes only for the intracellular loop …
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91246-plasmid-map-sequence-id-180624

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  39. TFORF2932

    Plasmid
    #144408
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2A (TFAP2A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144408-plasmid-map-sequence-id-353338

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  40. pLIX403-EYFP-ccdB-Zeo

    Plasmid
    #158565
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158565-plasmid-map-sequence-id-309880

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Showing: 2281 - 2320 of 170794 results