163,584 results

1. pHAGE2-EF1a-GFP1-10-IRES-Puro

   Plasmid

   #211467

   Purpose

   Lentivirus backbone expressing GFP1-10

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3037 GST-HPV-6 E6

   Plasmid

   #10851

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   HPV6b E6 (E6 Human papillomavirus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

3. IronFistMUT.

   Plasmid

   #243008

   Purpose

   Iron binding deficient IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   hemerythrin-like-mNeonGreen (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   H15A, H57A, E58A, E61A, H126A, E130A

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Iron Insensitive Control Construct 2

   Plasmid

   #243007

   Purpose

   Iron Insensitive Control Construct under the same expression promoter used in IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   mNeonGreen
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. IronFist

   Plasmid

   #243006

   Purpose

   Genetically encoded fluorescent iron reporter

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   Hemerythrin-like (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-myc-RIG-I

   Plasmid

   #240814

   Purpose

   Expresses myc-tagged RIG-I in mammalian cells

   Depositor

   AJ te Velthuis

   Insert

   RIG-I (RIGI Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX208-cytoFLARE1.0-TEV

   Plasmid

   #234512

   Purpose

   Transcriptional reporter for detecting calcium increase in HEK293T (TEVp); the same TEVp involved in FLiCRE2

   Depositor

   Wenjing Wang

   Article

   Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

   Insert

   EGFP-P2A-CaM-V5-TEVp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. CCLMPC_HA-NUP98::SETBP1

   Plasmid

   #237491

   Purpose

   Express HA-tagged NUP98::SETBP1 in dual promoter lentiviral vector

   Depositor

   Charles Mullighan

   Article

   Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

   Insert

   HA-NUP98::SETBP1 (NUP98, SETBP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MND/PGK

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF.DEST51-mTurquoise2

   Plasmid

   #215539

   Purpose

   Expression vector with the mTurquoise2 fluorescent protein

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

    Plasmid

    #239030

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse VGAT (Slc32a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBOBI-hPLD4-flag

    Plasmid

    #222641

    Purpose

    lentiviral vector for full length human PLD4 expression (with flag tag)

    Depositor

    David Nemazee

    Insert

    PLD4 (PLD4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGK-HA-PGRN

    Plasmid

    #234872

    Purpose

    Lentiviral plasmid expressing HA-tagged human progranulin.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    Progranulin (GRN Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX208-FLiCRE2-TF

    Plasmid

    #234523

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    CD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pgdh and Pxyl

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. peGFP-N1-Cofilin-S41E

    Plasmid

    #186748

    Purpose

    Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. peGFP-N1-Cofilin-S41A

    Plasmid

    #186749

    Purpose

    Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to alanine

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCherry-N1-Cofilin-S41E

    Plasmid

    #186751

    Purpose

    Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNLF1-N_DCAF15:1-600

    Plasmid

    #210958

    Purpose

    Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF15:M1-L600 (DCAF15 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pancortin scFv [K96/7]

    Plasmid

    #190497

    Purpose

    Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-mitoX4-mVenus

    Plasmid

    #185808

    Purpose

    Mitochondrial targetted (COX8) mVenus

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-mVenus
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. H2A-mTurquoise-CDC42-G12V-deltaCAAX

    Plasmid

    #176094

    Purpose

    Constitutively active, nuclear localized Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3puro-mGFP-ROCK2

    Plasmid

    #225472

    Purpose

    Express mEGFP-fusion protein of ROCK2

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX307 Luciferase

    Plasmid

    #117734

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSmartBAC-S

    Plasmid

    #107268

    Purpose

    Broad host range bacterial artificial chromosome vector

    Depositor

    Ron Godiska, Mark Liles

    Article

    Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pmScarlet3-LaminB_C1

    Plasmid

    #189776

    Purpose

    Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-LaminB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre-3G

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge