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161,570 results

Showing: 2901 - 2950 of 161570 results

  1. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

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  3. pFGL822

    Plasmid
    #58225
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    58225-plasmid-map-sequence-id-115839

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  13. mNeptune2-Tubulin-6

    Plasmid
    #56149
    Purpose
    Localization: Microtubules, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mNeptune2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56149-plasmid-map-sequence-id-209084

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  14. SmBiT-b4GALT1 Fusion Vector

    Plasmid
    #237043
    Purpose
    Express SmBiT-b4GALT1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    b4GALT1 (B4GALT1 Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237043-plasmid-map-sequence-id-471899

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  16. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  18. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

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  20. pCR4_miniFXN_8

    Plasmid
    #234361
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Article
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234361-plasmid-map-sequence-id-464762

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  21. pCR4_miniFXN_7

    Plasmid
    #234360
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Article
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234360-plasmid-map-sequence-id-464761

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  23. hSyn-eGFP-DDHD2

    Plasmid
    #232463
    Purpose
    Expresses GFP tagged DDHD2 lipase in neurons
    Depositor
    Timothy Ryan
    Insert
    DDHD2 (DDHD2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232463-plasmid-map-sequence-id-460234

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  28. mSirt1-GFP

    Plasmid
    #221511
    Purpose
    Fluorescently tagged mouse Sirt1-GFP
    Depositor
    Alessandro Ori
    Insert
    Sirt1 (Sirt1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221511-plasmid-map-sequence-id-439387

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  29. pJMP3666

    Plasmid
    #222352
    Purpose
    Expression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.
    Depositor
    Jason Peters
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PabstBR
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222352-plasmid-map-sequence-id-439071

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  31. pUBQ10-SNAP-GW

    Plasmid
    #209350
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    209350-plasmid-map-sequence-id-420465

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  32. pLCS110

    Plasmid
    #209331
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal StayGold fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209331-plasmid-map-sequence-id-420445

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  33. pLNHA-C1-HseIF4G_O

    Plasmid
    #147087
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147087-plasmid-map-sequence-id-289534

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  35. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

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  36. pPOU5F1-donor

    Plasmid
    #176367
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    POU5F1 (POU5F1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176367-plasmid-map-sequence-id-347873

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  37. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

    Plasmid
    #160628
    Purpose
    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160628-plasmid-map-sequence-id-321993

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  42. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185910-plasmid-map-sequence-id-363739

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  46. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  47. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55265-plasmid-map-sequence-id-475376

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Showing: 2901 - 2950 of 161570 results