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Plasmid#136902PurposeLentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)DepositorInsertFNLSHiFi
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174119PurposeExpression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)DepositorInsertsgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralTagsExpressionMutationPromotermU6 and UbCAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB6-pCMV
Plasmid#21258DepositorInsertHOXB6 (HOXB6 Human)
UseTagsFLAG_Pro TagExpressionBacterial and MammalianMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Sa-SchCas9
Plasmid#192137PurposeExpresses Sa-SchCas9, and cloning backbone for sgRNADepositorInsertSa-SchCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(R982A)
Plasmid#39487DepositorInsertARAP3(R982A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR982A = Rho GAP mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUHD Cdk2 WT HA
Plasmid#27650DepositorInsertCdk2 (CDK2 Human)
UseTagsHA (YDVPDYA)ExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM cGdf5 (CT#688)
Plasmid#13944DepositorInsertGdf5 (GDF5 Chicken)
UseTagsExpressionBacterialMutationAmino acids 285-411PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-3.2mDelta13
Plasmid#12992DepositorInsertFLIPglu F16A S112A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A, S112A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Lin28BdelN70
Plasmid#51374DepositorInsertLin28B (LIN28B Human)
UseTagsFLAGExpressionMammalianMutationN-terminal 70 amino acids deletionPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
Desmocollin2_tail-GST
Plasmid#36992DepositorInsertDsc2 (DSC2 Human)
UseTagsGSTExpressionBacterialMutationIntracellular domain of Dsc2 (nt 2,603–3,158)PromoterAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDY4
Plasmid#53589PurposeProduces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.DepositorInsertlcyE
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK1224
Plasmid#84221PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFPDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-014
Plasmid#78702PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUHD Cdk2 DN HA
Plasmid#27651DepositorInsertCdk2 (CDK2 Human)
UseTagsHA (YDVPDYA)ExpressionMammalianMutationD146N (Dominant Negative)PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAP1_Ta_Yr7_CDS_no_STOP
Plasmid#141093PurposeLevel 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codonDepositorInsertTa_Yr7
UseUnspecified; Level 0 module for golden gate cloni…TagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-HA-MmUbc6 mut/2775
Plasmid#11483DepositorInsertMmUbc6 (Ube2j2 Mouse)
UseTagsHAExpressionMammalianMutationcys 94 mutated to serPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
p068 pCS mTRPS1 (M)
Plasmid#11040DepositorInsertTRPS1 mut (Trps1 Mouse)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationTwo Cys residues at positions 917 and 920 in the …PromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
SRF 1-133 pGEX4T1
Plasmid#121101PurposeExpression of SRF fragment as a GST fusion proteinDepositorInsertSRF (SRF Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 1-133PromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV Lex VP16 (F442A) HA (P#1710)
Plasmid#14595DepositorInsertLexA
UseTagsHA and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-011
Plasmid#78699PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(R1155E)
Plasmid#39488DepositorInsertARAP3(R1155E) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR1155E = Ras binding domain mutationPromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-009
Plasmid#78696PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX3 (pcDNA3.1+)
Plasmid#75205PurposeMammalian expression of SLC24A3DepositorInsertSLC24A3 (SLC24A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AKAP13
Plasmid#73230PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAKAP13 (AKAP13 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336PromoterT7Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pPB-cT3G-cERP2-HOXB5
Plasmid#222537PurposePiggyBac transposon plasmid for doxycycline inducible expression of HOXB5DepositorInsertHOXB5 (HOXB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(1-573)
Plasmid#219683PurposeMammalian expression of CLIPf-KIF5A(1-573)DepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPfExpressionMammalianMutation1-573PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A
Plasmid#200790PurposeMammalian expression of CLIPf-KIF5ADepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPfExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBsVchDonor
Plasmid#203814PurposeDNA donor for transpositionDepositorInsertMini-Tn, aprE cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-HOXB5-DD-hygro
Plasmid#222582PurposePiggyBac transposon plasmid for Shield1-inducible expression of BCL2DepositorInsertHOXB5 (HOXB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERH-HOXB5
Plasmid#222584PurposePiggyBac transposon plasmid for doxycycline inducible expression of HOXB5, with hygromycin resistanceDepositorInsertHOXB5 (HOXB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-LAP2beta
Plasmid#222416PurposeTo express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLAP2beta (Tmpo Rat)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL686
Plasmid#207510PurposeePPE for prime editing in riceDepositorInsertzCas9 (H840A)-NC-M-MLV RT-deltaRH
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCitrine
Plasmid#214144PurposeVolume marker / Control sensorDepositorInsertmCitrine
UseTagsExpressionMammalianMutationPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M4
Plasmid#203320PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hMuc18 fused to Ig like C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M2
Plasmid#203318PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hMuc18 fused to Ig like C1-C3 of hB…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M1
Plasmid#203317PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hMuc18 fused to Ig like V2-C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B4
Plasmid#203316PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hBCAM fused to Ig like C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only