Addgene

We narrowed to 9,510 results for: Gnas

Showing: 2951 - 3000 of 9510 results

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Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 920-923

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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RnAT1aR-mCherry

Plasmid

#137782

Purpose

Visualization of At1aR

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat AT1a receptor (Agtr1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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coreR1 (384-1008)

Plasmid

#115646

Purpose

expression of truncated Rag1

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag1 core (384-1008) (Rag1 Mouse)

Use

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag1 (aa 384-1008)

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-PIP5K1A

Plasmid

#202743

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

S204A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Tmem98-FLAG

Plasmid

#124501

Purpose

Expresses FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 AT1R DRY-AAY

Plasmid

#45759

Depositor

Robert Lefkowitz, Laszlo Hunyady

Article

Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

Insert

AT1R DRY-AAY (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

changed amino acids 125-126 from DR to AA

Promoter

CMV

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pBAD-DiB2

Plasmid

#168801

Purpose

Fluorogen activating protein (FAP) DiB2

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB2

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1-MCS

Plasmid

#60608

Purpose

Cloning PAmCherry1

Depositor

Steven Chu, Xiaolin Nan

Article

Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

Insert

PAmCherry1

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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KG#59

Plasmid

#110929

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 Trx1 SSAAA

Plasmid

#98403

Purpose

Bacterial expression vector for Trx1 SSAAA

Depositor

Tobias Dick

Article

Schwertassek et al EMBO J. 2007 Jul 11;26(13):3086-97. Epub

Insert

Trx1 SSAAA (TXN Human)

Use

Tags

Expression

Bacterial

Mutation

C32S, C35S, C62A, C69A, C73A

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-human RNF146 RING-WWE (30-183)

Plasmid

#132611

Purpose

expresses human RNF146 RING-WWE (30-183) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 RING-WWE (30–183)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOME0015R

Plasmid

#178385

Purpose

Expresses KSHV ORF45

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 45

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

actin5C

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Ilmol

Plasmid

#120310

Purpose

A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

Depositor

Bradley Baker

Article

Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

Insert

Ilmol

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
CAG-RARa-VP16

Plasmid

#185559

Purpose

Ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJH3043

Plasmid

#175284

Purpose

Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16a mini-gene (daf-16 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Pdpy-30

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-SH3BP4

Plasmid

#46890

Purpose

expresses Myc tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 DN-Src

Plasmid

#19965

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

Src (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

K295M

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR9.924

Plasmid

#39539

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRRG203-notum

Plasmid

#99067

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea notum

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

notum in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-PRAS40 T246A

Plasmid

#15479

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Thr246 to Ala

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
XE152 GFP-XDpr-CS2+

Plasmid

#16770

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

GFP-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pJH2947

Plasmid

#132362

Purpose

Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-2 (ins-2 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
C-Ag15

Plasmid

#11868

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CLPIT Cry2PHR-mCh-Rac1

Plasmid

#42955

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet-Off promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt2B2

Plasmid

#35907

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-mPRAS40

Plasmid

#15480

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-HHARI(1-185)

Plasmid

#17341

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-185

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
C-Ag20(0)

Plasmid

#11866

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR9.1347

Plasmid

#39540

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-HA-hILP del RING

Plasmid

#25675

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Encodes aa 1-449 only. aa 450-497 have been dele…

Promoter

Available since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
NmMetQ WT

Plasmid

#129647

Purpose

Expresses the mature metQ gene from Neisseria meningitides, without the signal sequence

Depositor

Douglas Rees

Article

Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

Insert

metQ Gene

Use

Tags

10x Histidine Tag, Enterokinase, and pelB signal …

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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