We narrowed to 15,350 results for: genome
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Plasmid#64939PurposeExpresses gRNA against human CREB1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLC-ZsGreen-P2A-Puro
Plasmid#124302PurposeLentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoterDepositorInsertZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA1 TKIT donor
Plasmid#169418PurposeDonor DNA for TKIT knockin of SEP to GluA1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZBTB1
Plasmid#128235PurposeDonor vector for 3' FLAG tag of human ZBTB1DepositorInsertZBTB1 Homology arms
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B
Plasmid#69291PurposeGateway entry vector; single gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGG>nls-hCas9-nls
Plasmid#99138PurposeExpresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryosDepositorInserthumanized Cas9
UseCRISPRTagsExpressionMutationPromoterChicken beta actin (CAG)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB ωRNA-v13
Plasmid#219634Purposehuman U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloningDepositorInsertOgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x4
Plasmid#58768PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-luc2
Plasmid#138355PurposeGateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoterDepositorInsertluciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA13
Plasmid#160127PurposeTo express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-ADE2- HDV-TAMO
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.436
Plasmid#184953PurposeExpress -Eco1 RT and ncRNADepositorInsertEco1: ncRNA(wt) and RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH1
Plasmid#84451Purposeshuttle plasmid for sarA P1-dsRED expressionDepositorInsertDsRed.T3(DNT)
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-ZsGreen-P2A-Hygro
Plasmid#124301PurposeLentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoterDepositorInsertZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pxpr_502-wt-ltr
Plasmid#182647Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTRDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3
Plasmid#200631PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing padDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
BSP610
Plasmid#122255Purposeexpression vector for mtagBFP2 under EFT3 promoterDepositorInsertmtagBFP2
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mpCHECK2
Plasmid#167576PurposeThe mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
PE-Cas9
Plasmid#174851PurposeExpress PE-Cas9 in mammalian cellsDepositorInsertPE-Cas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaTagsExpressionMutationchanged lysine 219 to histidinePromoter2x35SAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET8
Plasmid#107439PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET8 (MET8 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221T7RNAP
Plasmid#167974PurposeExpresses T7RNAP in E. coliDepositorInsertT7RNAP (T7p07 Synthetic)
UseTagsExpressionMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDR-TetOn
Plasmid#183577PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterTet-OnAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgDonor_lentiGuide
Plasmid#184168PurposeLentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.DepositorInsertsgDonor
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pADH110
Plasmid#90982PurposeNAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.DepositorInsertsNAT 2 of 2
pSNR52
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_tdTFlag
Plasmid#46024DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTagstdTomatoExpressionMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA787
Plasmid#186242Purposesoc-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145
Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKDR-CBh
Plasmid#183557PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221AID-T7RNAP
Plasmid#167977PurposeExpresses fusion protein AID-T7RNAP in E. coliDepositorInsertAID-T7RNAP
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH102
Plasmid#128207PurposesgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Simple-gRNA backbone
Plasmid#51306PurposegRNA expression plasmidDepositorTypeEmpty backboneUseCRISPR; Xenopus expressionTagsExpressionMutationPromoterT7Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
p221z-dCAS9p-t35s
Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
UseTagsExpressionWormMutationPromoterrps-27 promoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-hsp70
Plasmid#71500PurposeflySTARR-seq screening vector with hsp70 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDU1
Plasmid#169817PurposepSpCas9(BB)-2A-tCD19 (pDU1)DepositorInserttCD19
UseTagsfusion proteinExpressionMammalianMutationPromoterCbhAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH103
Plasmid#128208PurposecrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D
Plasmid#69293PurposeGateway entry vector; single gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneUseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only