We narrowed to 5,536 results for: Chia
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CMYA5T2/pDEST17
Plasmid#98118PurposeExpress CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteriaDepositorInsertCMYA5 (CMYA5 Human)
UseTags6X-HisExpressionBacterialMutationrs10043986 (NP_705838.3:p.Pro4063Leu)PromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5T/pACT2
Plasmid#97209PurposeCMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationrs10043986 (NP_705838.3:p.Pro4063Leu)PromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa02_2817
Plasmid#49654DepositorInsertECF02_2817
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CSII-PPM1H-mApple
Plasmid#198473PurposeMammalian expression of PPM1H-mAppleDepositorInsertPPM1H-mApple (PPM1H Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
DYRK1A/pTRE3G-FL-hXIST
Plasmid#149608PurposeExpress doxycycline-inducible full length of human XIST cDNADepositorInsertX-inactive specific transcript (XIST Human)
UseTagsExpressionMammalianMutationPromoterpTRE3GAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264-deltaLRR-His6
Plasmid#220215PurposeBacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationc.4-75del / p.2-24delPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUG-Pten
Plasmid#140732PurposeLentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion proteinDepositorInsertGFP-Pten (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhUbiCAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-sufABCDSE
Plasmid#195056PurposeExpresses SUF operon from E. coli from IPTG-inducible promoterDepositorInsertsufABCDSE
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His-mPer1
Plasmid#219421PurposeExpresses 3873 bp of mPer1 coding sequence in E. coli.DepositorInsertmPer1 (Per1 Mouse)
UseTagsHis and V5ExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire_blastocidin
Plasmid#174103PurposepGreenFire MPRA plasmid for cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CYFIP1.mCherry
Plasmid#122051PurposeExpresses human CYFIP1 in mammalian cells.DepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP1.GFP
Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H sfGFP-R5 AmpR
Plasmid#225139PurposeRecombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 protein fused to silaffin-R5 and sfGFP
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5 TEX264wt-cGFP
Plasmid#220211PurposeMammalian expression of wild type TEX264 fused at C-terminus to GFPDepositorInsertTEX264 (TEX264 Human)
UseTagsStrep-strep-HAExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET14b His Rab8a Q67L
Plasmid#186014PurposeHis Rab8a Q67LDepositorInsertHis Rab8a Q67L (RAB8A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
c1GFP-W53XKir7.1
Plasmid#107183PurposeGenerates Nonsense mutant cloneDepositorInsertKCNJ13 (KCNJ13 Human)
UseTagsEGFPExpressionMammalianMutationW53XPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF16B tail 458-1317
Plasmid#134624PurposeMammalian expression of human KIF16B 458-1317DepositorInsertKIF16B (KIF16B Human)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 457 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_CEACAM6-His
Plasmid#149337Purposefor eukaryotic expression and purification of CEACAM6-His6DepositorInsertCEACAM6 (CEACAM6 Human)
UseTagsIL-2 signal peptide and polyhistidineExpressionMammalianMutationpoint mutation (GGA to GCA) to replace 'G-g…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab8A
Plasmid#198470Purposelentiviral expression of GFP-Rab8ADepositorInsertGFP-Rab8A (RAB8A Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
UseTagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
UseTags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 2
Plasmid#198492Purposelentiviral stable expression of mMyh9 gRNA 2DepositorInsertmMyh9 (Myh9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 1
Plasmid#198491Purposelentiviral stable expression of mMyh9 gRNA 1DepositorInsertmMyh9 (Myh9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NDP52
Plasmid#135296Purposestably express GFP-tagged NDP52 for CID induced mitophagyDepositorInsertNDP52 (CALCOCO2 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1
Plasmid#19132DepositorInsertMcl-1 promoter (MCL1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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FUG-T2A-Pten
Plasmid#140738PurposeLentiviral vector to overexpress human PTEN and GFPDepositorInsertGFP-PTA-PTEN (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB.GFP-KIF1A tail 395-1698
Plasmid#134618PurposeMammalian expression of mouse KIF1A 395-1698DepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 394 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1C tail 400-1103
Plasmid#134621PurposeMammalian expression of human KIF1C 400-1103DepositorInsertKIF1C (KIF1C Human)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
