159,237 results

1. pEGFP-HP1alpha

   Plasmid

   #179701

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PLKO-mcherry-luc-puro-TRPM1 cDNA

   Plasmid

   #29782

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   TRPM1 (TRPM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO-RB1-shRNA19

   Plasmid

   #25640

   Purpose

   3rd generation lentiviral vector

   Depositor

   Todd Waldman

   Article

   Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

   Insert

   RB1-shRNA 19 (RB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cbh_v5 AAV-ABE N-terminal

   Plasmid

   #137177

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-ABE N-terminal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPIPL12-FnCas12a

   Plasmid

   #199563

   Purpose

   Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   Fncas12a
   

   Use

   CRISPR and Synthetic Biology; Tetracycline induci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   COtetR-PIPL12

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPfetFn-Target_v2

   Plasmid

   #199564

   Purpose

   gRNA expression for FnCas12a in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   SacB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pthl_fU

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-HP1alpha

   Plasmid

   #179703

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGI3EM17

   Plasmid

   #210569

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-hPar2

    Plasmid

    #53228

    Purpose

    mammalian expression of human Par2

    Depositor

    Shaun Coughlin

    Article

    Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

    Insert

    Par2 (F2RL1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiCRISPRv2 RFP670

    Plasmid

    #187646

    Purpose

    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

    Depositor

    Martin Carroll

    Insert

    RFP 670
    

    Use

    Lentiviral

    Tags

    RFP670 and sgRNA cloning site

    Expression

    Mutation

    Promoter

    EFS (P2A)

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSET his-eGFP

    Plasmid

    #113551

    Purpose

    For bacterial expression and purification of eGFP

    Depositor

    Jeanne Stachowiak

    Article

    Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

    Insert

    his-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A206K

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LZRS-hNEDD9_IresGFP

    Plasmid

    #21962

    Depositor

    Lynda Chin

    Article

    Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

    Insert

    NEDD9 (NEDD9 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pLIC-SM-HA

    Plasmid

    #111188

    Purpose

    High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster HA Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. CAV1-P132L-mEGFP

    Plasmid

    #27708

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    P132L

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUA66 PrpsL GFP

    Plasmid

    #105606

    Purpose

    Expresses GFP under PrpsL

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsL

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdCas9- AsiA_m2.1

    Plasmid

    #158065

    Purpose

    Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

    Depositor

    Harris Wang

    Article

    Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

    Insert

    dCas9-AsiA_m2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-mFgf21pro1-luc

    Plasmid

    #101797

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV-CAG-oScarlet-WPRE-BC1-pA

    Plasmid

    #220765

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge