173,291 results

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pZT-C13-L1

Plasmid

#62196

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Left CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmStayGold_I3

Plasmid

#239336

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mStayGold

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJQ200SK

Plasmid

#78497

Purpose

Suicide vector for gene replacement

Depositor

Michael Hynes

Article

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide plasmid

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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phCMV-coGALVenv-C4070A

Plasmid

#172217

Purpose

Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

Depositor

Boris Fehse

Article

Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

Insert

Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)

Expression

Mammalian

Mutation

Carries C-terminus of amphotropic MLV strain 4070…

Promoter

CMV immediate early promoter

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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YAP/TAZ tdTomato TEAD Binding Reporter

Plasmid

#228554

Purpose

Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

Depositor

Cole DeForest

Article

Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

Insert

tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

GTIIC TEAD-Binding Responsive Element

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-Halo-RNaseL

Plasmid

#250856

Purpose

lentiviral vector for expressing Halo-tagged RNase L

Depositor

Britt Glaunsinger

Insert

RNASEL (RNASEL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR

Plasmid

#250291

Purpose

PiggyBac integration of 3 transcription factors (SPI1, CEBPA, FLI1) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

SPI1-T2A-CEBPA-P2A-FLI1

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ack

Plasmid

#62654

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-evoTnsAB

Plasmid

#234729

Purpose

Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

evoPseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCasAb-apr

Plasmid

#121998

Purpose

Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP

Plasmid

#37825

Purpose

AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Has Service

AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-evoTnsC

Plasmid

#234730

Purpose

Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

evoPseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-Cas6

Plasmid

#234725

Purpose

Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseCas6

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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V5-miniTurbo-NES_pCDNA3

Plasmid

#107170

Purpose

expresses V5-tagged miniTurbo in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur

Plasmid

#60507

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic cpGFP

Plasmid

#215709

Purpose

Expresses cpGFP in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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