12,913 results

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pCDNA HA FAK Y397F

Plasmid

#50503

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pSirt6-GFP

Plasmid

#20275

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-6A

Plasmid

#51251

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848,923,956 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pk335-CARGO-6mer-70kb-DSF

Plasmid

#227498

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 70kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#1-puro

Plasmid

#231977

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SKM

Plasmid

#136551

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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q4wt

Plasmid

#24502

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha

Tags

HA (see comments)

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only
FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMK467 (dTAG-BromoTag-mAID-EGFP)

Plasmid

#214393

Purpose

dTAG-BromoTag-mAID-EGFP reporter (TOL2)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BromoTag-mAID-EGFP reporter

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Myo1E-pEGFP

Plasmid

#124658

Purpose

Mouse myosin-1e fused with EGFP

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Myo1e (Myo1e Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-mSlug

Plasmid

#34584

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Insert

Slug (Snai2 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-CreERT2 sgRipk4 v3

Plasmid

#158048

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
p3xFLAG-mSnail

Plasmid

#34583

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Insert

Snail (Snai1 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLV Nup62-V5

Plasmid

#175116

Purpose

Lentiviral expression of mouse Nup62-V5

Depositor

Larry Gerace

Insert

Nup62 (Nup62 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Plxna4-Fc-His

Plasmid

#72125

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-mH33B-mClover3

Plasmid

#171496

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

H3.3B (H3f3b Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ack1-pmCherryC1

Plasmid

#27684

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ack1 (Tnk2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-ms-Sox17

Plasmid

#50781

Purpose

retroviral expression of mouse Sox17

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox17 (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-HA-mTmod3-WT

Plasmid

#70752

Purpose

N-terminal-HA-tagged mouse Tropomodulin3, WT

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-Gaolf-Integration

Plasmid

#129456

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

G alpha olf (Gnal Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Mouse Ism1 with C-terminal Myc-6X-his tag

Plasmid

#173046

Purpose

Expresses Ism1 in mammalian cells

Depositor

Katrin Svensson

Article

Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

Insert

Ism1 (Ism1 Mouse)

Expression

Mammalian

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

Plasmid

#156434

Purpose

Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

CTCF, rtta3G, TetO3G (Ctcf Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

All zinc fingers mutated

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-Bmal1

Plasmid

#239045

Purpose

Overexpression of Bmal1

Depositor

Qingchun Tong

Article

Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

Insert

mArntl (Bmal1 Mouse)

Use

AAV

Promoter

Bmal1

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Tags

His and V5

Expression

Bacterial and Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-VASP-5

Plasmid

#58027

Purpose

Localization: Actin/Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

VASP

Tags

mTagRFP

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EGR-1 TurboRFP

Plasmid

#52306

Purpose

Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Varma et al Lab Chip. 2015 Feb 4.

Insert

EGR-1 Promoter (Egr1 Mouse)

Tags

TurboRFP

Expression

Mammalian

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-mTRAF2

Plasmid

#174736

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

TRAF2 (Traf2 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRC54-DHX37-GFP

Plasmid

#192149

Purpose

express DHX37-GFP

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Insert

DHX37-GFP (Dhx37 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc2 mAbp1

Plasmid

#26719

Depositor

Anna Huttenlocher

Article

Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

Insert

Actin-binding Protein-1 (Dbnl Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL-p27UTR

Plasmid

#20881

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p27 3'UTR (Cdkn1b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
Plxnb2(L)-Fc-His

Plasmid

#72128

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nxph4-2A-CreERT2 Targeting Vector

Plasmid

#62219

Purpose

Targets CreERT2 to the mouse Nxph4 stop codon

Depositor

Hongkui Zeng

Article

Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

Insert

Nxph4-2A-CreERT2 (Nxph4 Mouse, Bacteriophage)

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

Mouse Nxph4

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Bmi1 shRNA1

Plasmid

#21576

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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12,913 results