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Showing: 2641 - 2680 of 158307 results

  1. pcDNA3-N-I-N-CAAX

    Plasmid
    #41686
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    Use
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41686-plasmid-map-sequence-id-55893

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  4. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Use
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

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  6. pFGL821

    Plasmid
    #58223
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    hygromycin B phosphotransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58223-plasmid-map-sequence-id-89704

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  7. ECFP-Peroxisomes-0

    Plasmid
    #54548
    Purpose
    Localization: Peroxisomes, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Peroximal Targeting Signal 1 (PTS1)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. FGB

    Plasmid
    #52030
    Purpose
    Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FGB (FGB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 492, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52030-plasmid-map-sequence-id-80105

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  12. LSB-hsa-let-7a-5p

    Plasmid
    #103146
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103146-plasmid-map-sequence-id-213119

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  13. TFORF0340

    Plasmid
    #141583
    Purpose
    Lentiviral vector for overexpressing the MECOM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MECOM (MECOM Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141583-plasmid-map-sequence-id-400390

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  17. pCfB2188

    Plasmid
    #67296
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67296-plasmid-map-sequence-id-124518

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  24. pBK092

    Plasmid
    #183146
    Purpose
    pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression
    Depositor
    Daan Swarts
    Article
    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.
    Insert
    CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
    Use
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    183146-plasmid-map-sequence-id-360089

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  26. pcDNA3.1-MCS-mCherry_AB

    Plasmid
    #148305
    Purpose
    Mammalian Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148305-plasmid-map-sequence-id-426368

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  27. R777-E143 Hs.NFKB1

    Plasmid
    #70427
    Purpose
    Gateway ORF clone of human NFKB1 [NM_003998.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NFKB1 (NFKB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70427-plasmid-map-sequence-id-125876

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  28. NEDD4

    Plasmid
    #25141
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4.0001.0152 (NEDD4 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pBS L30 mRuby3-Rab27A

    Plasmid
    #166432
    Purpose
    Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab27A
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166432-plasmid-map-sequence-id-333547

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Showing: 2641 - 2680 of 158307 results