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Showing: 121 - 140 of 8568 results

  3. pBiFC-VN173

    Plasmid
    #22010
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VN173
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Venus residus 1-172
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22010-plasmid-map-sequence-id-354487

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  5. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  6. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

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  8. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  9. SV40 1: pBSSVD2005

    Plasmid
    #21826
    Depositor
    David Ron
    Insert
    SV40 Large T (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    likely expresses aa109-708 of the large T antigen
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21826-plasmid-map-sequence-id-352432

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  10. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179390-plasmid-map-sequence-id-451660

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  15. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin and U6
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

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Showing: 121 - 140 of 8568 results