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Plasmid#148478PurposeMammalian Expression of HsMex3BDepositorInsertHsMex3B (MEX3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pscMS2(WTx2)-EGFP
Plasmid#140258PurposeMS2CP-dependent translational repressionDepositorInsertTwo MS2CP-binding sites embedded in scaffold
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsMex3C_AD
Plasmid#148481PurposeMammalian Expression of HsMex3CDepositorInsertHsMex3C (MEX3C Human)
UseTagsExpressionMammalianMutationL11P in Mex3CPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPH1_Z
Plasmid#148120PurposeMammalian Expression of HshnRNPH1DepositorInsertHshnRNPH1 (HNRNPH1 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPA1_Z
Plasmid#148112PurposeMammalian Expression of HshnRNPA1DepositorInsertHshnRNPA1 (HNRNPA1 Human)
UseTagsExpressionMammalianMutationone non silent mutation I136V compared to the tra…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGH156_MS2-AID-Hygro
Plasmid#85415Purposelentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable markerDepositorInsertMS2-AID and Hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113344PurposeExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113342PurposeExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertdCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5 PMS2-134
Plasmid#16476DepositorInsertPMS2 1-134 (PMS2 Human)
UseTagsExpressionMammalianMutationCodons 1-134PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNeuroguidin_AA
Plasmid#148237PurposeMammalian Expression of HsNeuroguidinDepositorInsertHsNeuroguidin (NGDN Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTP112 (pMS26_miniTn6900(KanR))
Plasmid#164260PurposeDelivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.DepositorInsertminiTn6900 element
UseBacterial delivery vector for tn7 att siteTagsExpressionMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRRP6_Z
Plasmid#148131PurposeMammalian Expression of HsRRP6DepositorInsertHsRRP6 (EXOSC10 Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence of is…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159
Plasmid#170170PurposeLentiviral Expression of EF1a.SLC6A1.WTDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH361 Hspa8 MS2 HA
Plasmid#168218PurposeMammalian expression of heat shock 70 kDa protein 8 fused to the MS2 dimer and C-terminal HA tagDepositorInsertHspa8
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
sm-9xGCN4-Renilla-MS2
Plasmid#178009PurposeExpresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cellsDepositorInsertsm-9xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD_NLS
Plasmid#124704PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLSDepositorInsertMCP_ADAR2 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-HsHelz_AG
Plasmid#148805PurposeMammalian Expression of HsHelzDepositorInsertHsHelz (HELZ Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164
Plasmid#170169PurposeLentiviral Expression of EF1a.SLC6A1.WT.EGFPDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS25: pTns(PmcCAST)
Plasmid#168158PurposeInducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMEH60 Lamp2b MS2 HA
Plasmid#168209PurposeMammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD_NLS
Plasmid#124625PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLSDepositorInsertMCP_ADAR1 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorInsertMS2
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-Scaffold
Plasmid#78906PurposeVector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_3_TOPO_TA-ms2-VP64
Plasmid#79371PurposeRecruits VP64 to a compatible gRNA for transcriptional activationDepositorInsertMS2-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNX24-RFP-MS2SL
Plasmid#227819PurposeExpresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance geneDepositorInserts3xHA-RFP-24xMS2SL
Neo resistance
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA-MS2
Plasmid#207624PurposeExpresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)DepositorInsertcrRNA-MS2
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-2xMS2
Plasmid#212627PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only