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pJL-SaKKH-BE3

Plasmid

#85170

Purpose

Expresses SaKKH-BE3 in mammalian cells [Edit]

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

SaKKH-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NLS-GFP

Plasmid

#17300

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-GFP-NLS (Pvalb Rat)

Use

Tags

Myc and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Use

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Use

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR1a

Plasmid

#17926

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(CTRL)-CMV-eGFP

Plasmid

#194017

Purpose

Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(CTRL)
eGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Article

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Use

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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PSD-95 FLAG

Plasmid

#15463

Depositor

Wei-dong Yao

Article

Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

Insert

PSD-95 (Dlg4 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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BE4

Plasmid

#100802

Purpose

Expresses BE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG8-GFP KI #2

Plasmid

#131474

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
VAMP2-pHuji

Plasmid

#105289

Purpose

VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2 (Vamp2 Rat)

Use

Tags

pHuji

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3-

Plasmid

#68822

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6p1-GST-ArcFL

Plasmid

#119877

Purpose

Bacterial Expression construct for protein production and purification

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP2A-mNeonGreen

Plasmid

#228525

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

LAMP2 (Lamp2 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Shank2-HA /pCDNA3

Plasmid

#197334

Purpose

Plasmid expressing an antigen targeting Shank2

Depositor

Morgan Sheng

Insert

Shank2 (Shank2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cx43-msfGFP

Plasmid

#69007

Purpose

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Gja1 (Gja1 Rat)

Use

Tags

monomerized superfolder GFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-LAMP1-phLuorin-N-8

Plasmid

#54918

Purpose

Localization: Lysosome Membrane, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

LAMP1 phLuorin

Use

Tags

mApple

Expression

Mammalian

Mutation

Lysosomes-20 double labeled with phLuorin

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HA GST PreScission p70 S6K1

Plasmid

#15511

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

p70 S6K1 (Rps6kb1 Rat)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
Green-Camuialpha

Plasmid

#26933

Depositor

Ryohei Yasuda

Article

Lee et al Nature. 2009 Mar 19. 458(7236):299-304.

Insert

REACh-CaMKIIa-mEGFP

Use

Tags

REACh and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
cacna2d1 pcDNA3

Plasmid

#206103

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit

Depositor

Annette Dolphin

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)

Plasmid

#215004

Purpose

Shuttling vector for an autophagy (LC3) reporter

Depositor

Wade Harper

Insert

MAP1LC3B (MAP1LC3B Rat)

Use

Gateway

Tags

HaloTag(7)-mGFP

Expression

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-HA-S6K1-WT

Plasmid

#8984

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-EPSIN 1

Plasmid

#22228

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

please see depositor comment below

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-HA-S6K1-KR

Plasmid

#8985

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K100R, kinase-inactive

Promoter

Available since

Jan. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Use

Tags

eGFP

Expression

Mutation

Promoter

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-T-rnVAPA-1-125

Plasmid

#13396

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2009

Availability

Academic Institutions and Nonprofits only

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