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Showing: 301 - 350 of 17683 results

  8. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Use
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  32. SpMTA Purple Sea Urchin Metallothionein

    Plasmid
    #200292
    Purpose
    Bacterial expression plasmids for production of recombinant Purple Sea Urchin Strongylocentrotus purpuratus Metallothionein
    Depositor
    Artur Krężel
    Article
    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.
    Insert
    Gene of metallothionein with Chiting Binding Domanin-Intein
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    200292-plasmid-map-sequence-id-394816

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  39. pLenti-C-Myc-DDK-IRES-Puro METTL3

    Plasmid
    #200724
    Purpose
    Lentiviral expression of Flag-Tagged human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200724-plasmid-map-sequence-id-401795

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  40. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  42. Ratiometric FPX sensor for ERK kinase activity

    Plasmid
    #60974
    Purpose
    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A-WW domain-ERK substrate-ddFP B
    Use
    Tags
    ERK substrate is before ddFP copy B, WW domain is…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  45. SmtA Synechococcus elongatus PCC 7942 Metallothionein

    Plasmid
    #200298
    Purpose
    Bacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 Metallothionein
    Depositor
    Artur Krężel
    Article
    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.
    Insert
    Gene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200298-plasmid-map-sequence-id-394900

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  49. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118581
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promotoer
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118581-plasmid-map-sequence-id-228549

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  50. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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Showing: 301 - 350 of 17683 results