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177,146 results

Showing: 3451 - 3500 of 177146 results

  6. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Publication
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    80478-plasmid-map-sequence-id-404034

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  9. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Publication
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    60444-plasmid-map-sequence-id-266122

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  10. p11-LacY-wtx1

    Plasmid
    #69056
    Purpose
    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.
    Depositor
    Huimin Zhao
    Publication
    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15
    Inserts
    ccdB (ccdB )
    lacY (lacY )
    Tags
    A177C
    Promoter
    pBAD and pLac
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    69056-plasmid-map-sequence-id-372941

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  13. mEmerald-TOMM20-N-10

    Plasmid
    #54282
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    15 Articles
    54282-plasmid-map-sequence-id-257386

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  16. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid
    #50479
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has service
    AAV5
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    50479-plasmid-map-sequence-id-166303

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  17. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid
    #46913
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-p65AD-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    2xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    46913-plasmid-map-sequence-id-66611

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  18. pcDNA5-EGFP-AID-BubR1

    Plasmid
    #47330
    Purpose
    for plasmid integration using the Flp-In system. AID at N-terminal
    Depositor
    Don Cleveland
    Publication
    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    Tags
    AID and GFP
    Expression
    Mammalian
    Mutation
    siRNA resistant
    Available since
    Sept. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    47330-plasmid-map-sequence-id-511185

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  24. pLenti6-MK1-EHMT2-V5

    Plasmid
    #31113
    Depositor
    Bernard Futscher
    Insert
    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Stop codon mutated to Ala
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    31113-plasmid-map-sequence-id-504129

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  30. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    22028-plasmid-map-sequence-id-213358

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  40. RSV-Sp1

    Plasmid
    #12098
    Depositor
    Robert Tjian
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sp1 (SP1 Human)
    Expression
    Mammalian
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    12098-plasmid-map-sequence-id-243848

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  41. pCMV-PE7-P2A-GFP

    Plasmid
    #222996
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222996-plasmid-map-sequence-id-442553

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  43. pCMV-Hs.cargo(Cas9)

    Plasmid
    #174864
    Purpose
    Expresses human Peg10 cargoRNA encoding SpCas9
    Depositor
    Feng Zhang
    Publication
    Segel et al Science. 2021 Aug 20;373(6557):882-889.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 flanked by HsPeg10 UTRs
    Tags
    HA-SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174864-plasmid-map-sequence-id-342428

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  49. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    100901-plasmid-map-sequence-id-197197

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Showing: 3451 - 3500 of 177146 results