Skip to main content
Addgene
Showing: 3451 - 3500 of 9181 results

  3. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

    Enlarge

  4. pTH846-gst-dissc

    Plasmid
    #96907
    Purpose
    Plasmid for yeast expression of GST encoded by non-preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon dis-optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96907-plasmid-map-sequence-id-206054

    Enlarge

  5. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

    Enlarge

  6. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

    Enlarge

  7. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

    Enlarge

  15. pBP-BBa_B0012

    Plasmid
    #72997
    Purpose
    BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Bba_B0012 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72997-plasmid-map-sequence-id-447483

    Enlarge

  16. pBP-BBa_B0015

    Plasmid
    #72998
    Purpose
    BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    BBa_B0015 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72998-plasmid-map-sequence-id-447484

    Enlarge

  17. pTU2_2b

    Plasmid
    #73011
    Purpose
    Secondary module (BpiI) site in pTU2b backbone
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Secondary module + Golden Gate pTU2b BsmBI destination site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    73011-plasmid-map-sequence-id-133862

    Enlarge

  18. pBP-ORF-eCFP

    Plasmid
    #72961
    Purpose
    Level 0 Entry vector" ORF - eCFP"
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Enhanced cyan fluorescent protein (eCFP)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72961-plasmid-map-sequence-id-447498

    Enlarge

  19. pBP-ORF-mCherry

    Plasmid
    #72962
    Purpose
    Level 0 Entry vector" ORF - mCherry "
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    mCherry fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72962-plasmid-map-sequence-id-447500

    Enlarge

  20. pBP-SJM902

    Plasmid
    #72967
    Purpose
    Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM902 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  21. pBP-SJM903

    Plasmid
    #72968
    Purpose
    Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM903 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. pBP-SJM905

    Plasmid
    #72969
    Purpose
    Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM905 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pBP-SJM906

    Plasmid
    #72970
    Purpose
    Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM906 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pBP-SJM908

    Plasmid
    #72971
    Purpose
    Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM908 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pBP-SJM910

    Plasmid
    #72972
    Purpose
    Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM910 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pBP-SJM912

    Plasmid
    #72974
    Purpose
    Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM912 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pBP-SJM914

    Plasmid
    #72975
    Purpose
    Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM914 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. pBP-SJM915

    Plasmid
    #72976
    Purpose
    Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM915 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pBP-T7

    Plasmid
    #72977
    Purpose
    T7 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72977-plasmid-map-sequence-id-447501

    Enlarge

  30. pBP_BBa_B0034

    Plasmid
    #72980
    Purpose
    Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Bba_B0034 RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72980-plasmid-map-sequence-id-133825

    Enlarge

  31. pBP-Tag_linker

    Plasmid
    #72982
    Purpose
    N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    N-terminal linker with pET-RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72982-plasmid-map-sequence-id-133829

    Enlarge

  32. pBP-HexHis

    Plasmid
    #72983
    Purpose
    Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Polyhistidine (HHHHHH) N-terminal tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72983-plasmid-map-sequence-id-133831

    Enlarge

  33. pTU1-C-lacZ

    Plasmid
    #72937
    Purpose
    Level 1 Destination vector - Position C
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - C site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72937-plasmid-map-sequence-id-447510

    Enlarge

  34. pTU1-D-lacZ

    Plasmid
    #72938
    Purpose
    Level 1 Destination vector - Position D
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72938-plasmid-map-sequence-id-447511

    Enlarge

  35. pTU1-D-RFP

    Plasmid
    #72942
    Purpose
    Level 1 Destination vector - Position D
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72942-plasmid-map-sequence-id-447523

    Enlarge

  36. pTU1-D1-RFP

    Plasmid
    #72943
    Purpose
    Level 1 Destination vector - Position D1
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - D1 site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72943-plasmid-map-sequence-id-447512

    Enlarge

  37. pTU1-E-RFP

    Plasmid
    #72944
    Purpose
    Level 1 Destination vector - Position E
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 1 - E site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Beta-lactamase - G717C (nucleotide) - silent muta…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72944-plasmid-map-sequence-id-447513

    Enlarge

  38. pBP-ORF-eGFP

    Plasmid
    #72960
    Purpose
    Level 0 Entry vector" ORF - eGFP "
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Enhanced green flourescent protein (eGFP)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72960-plasmid-map-sequence-id-447499

    Enlarge

  49. pJK591

    Plasmid
    #71589
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine and aspartate-177 …
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71589-plasmid-map-sequence-id-130927

    Enlarge

Showing: 3451 - 3500 of 9181 results