We narrowed to 11,416 results for: 112

1. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLEGFP-S727A-STAT3

   Plasmid

   #71446

   Purpose

   Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   S727A

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. delCMV-mCherry-3x-p67phox-GBD

   Plasmid

   #214141

   Purpose

   Rac activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xp67phox-GBD (NCF2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE-hLin28B

   Plasmid

   #26358

   Depositor

   George Daley

   Article

   Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

   Insert

   lin-28 homolog B (LIN28B Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE-3FLAG-RAB11A

   Plasmid

   #176489

   Purpose

   For lentiviral transduction of 3FLAG-RAB11A

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-ALK2 wt

   Plasmid

   #80870

   Purpose

   mammalian expression of ALK2 WT

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK2 (ACVR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-FLAG-puro-PTPIP51(1-470)

   Plasmid

   #170536

   Purpose

   Expresses PTPIP51(1-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE HA Chk2 WT

   Plasmid

   #41901

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   CHK2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2895

    Plasmid

    #144355

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MTA1 (MTA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pORANGE CACNG8-GFP KI #2

    Plasmid

    #131474

    Purpose

    Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng8 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-C1-IFT43

    Plasmid

    #218735

    Purpose

    Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    IFT43 (IFT43 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Kif5A-CIBN

    Plasmid

    #102251

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

17. pSG5-HA-p300-DY1399

    Plasmid

    #89095

    Purpose

    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300-D1399Y mutant (EP300 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p300-DY1399

    Promoter

    SV40

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CHRM1-Tango

    Plasmid

    #66248

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CHRM1 (CHRM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. FLAG-MED7

    Plasmid

    #15416

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED7 (MED7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. DPYD-FLAG

    Plasmid

    #61616

    Purpose

    The human DPYD was used to study the effect of DPYD expression on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (DPYD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A

    Plasmid

    #100156

    Purpose

    Expresses KIF20A

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-Kif5a-Myc

    Plasmid

    #186613

    Purpose

    Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Heavy Chain isoform 5A (KIF5A Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. gh69

    Plasmid

    #106740

    Purpose

    expression of gRNA targeting B3GNT3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT3 (B3GNT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ELK3_pet28a

    Plasmid

    #131651

    Purpose

    Bacterial expression of ETS gene ELK3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GPR150-Tango

    Plasmid

    #66326

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR150 (GPR150 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiCRISPRv2 Neo sgTREX1

    Plasmid

    #127645

    Purpose

    Knock-out of human TREX1 with NeoR

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    TREX1 sgRNA (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUDC175

    Plasmid

    #103019

    Purpose

    S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Use

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK5-myc-Miro1 K427V

    Plasmid

    #47892

    Purpose

    Expresses myc tagged Miro1 K427 constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 K427V (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K427V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pLEGFP-K685R-STAT3

    Plasmid

    #71449

    Purpose

    Retroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    K685R

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

34. TFORF0473

    Plasmid

    #142637

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP140 (SP140 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-SP140

    Plasmid

    #65394

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP140 (SP140 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

36. nLYN267-gs10-NLS-VPR

    Plasmid

    #188265

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nLYN267 (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. K15-EGFP

    Plasmid

    #44266

    Depositor

    George Cotsarelis

    Article

    Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep

    Insert

    K15 promoter (-4.8) (Krt15 Mouse)
    

    Use

    Mouse Targeting; Fluorescence microscopy

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains the murine K15 promoter (-4.8) fragment

    Promoter

    murine K15 promoter (-4.8) fragment

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pEGFP-N1-PHD2

    Plasmid

    #21401

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pEBB HA CCDC22

    Plasmid

    #74903

    Purpose

    Express CCDC22 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

    Insert

    CCDC22 (CCDC22 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. myc-KIF5A

    Plasmid

    #127616

    Purpose

    N-terminal myc-tagged mouse KIF5A

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLEGFP-K49R-STAT3

    Plasmid

    #71447

    Purpose

    Retroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    K49R

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pLEGFP-K140R-STAT3

    Plasmid

    #71448

    Purpose

    Retroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    K140R

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

43. TFORF1564

    Plasmid

    #143521

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF682 (ZNF682 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TFORF1712

    Plasmid

    #141869

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DPRX (DPRX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. EF1a_MESP1_P2A_Hygro_Barcode

    Plasmid

    #120459

    Purpose

    Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMXS-IRES-Blast CHKA

    Plasmid

    #218540

    Purpose

    human CHKA cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CHKA (CHKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. gh136

    Plasmid

    #106806

    Purpose

    expression of gRNA targeting EXT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    EXT2 (EXT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRK5-myc-Miro1 S432N

    Plasmid

    #47893

    Purpose

    Expresses myc tagged Miro1 S432N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 S432N (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S432N, dominant negative mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

49. tetO-MESP1

    Plasmid

    #170694

    Purpose

    doxycycline-inducible overexpression of MESP1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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