We narrowed to 23,700 results for: Spr

1. pCas3-Sm

   Plasmid

   #208001

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. OmEF1aCas9P2ApuroSB

   Plasmid

   #165484

   Purpose

   Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

   Depositor

   Dietmar Kueltz

   Article

   Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

   Insert

   Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mutation

   Promoter

   Oreochromis mossambicus EF1 alpha promoter (OmEF1…

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH-CTX1-rhadCas9-native

   Plasmid

   #129392

   Purpose

   Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine, Histidine 840 to Alanine

   Promoter

   PrhaBAD

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

   Plasmid

   #130639

   Purpose

   Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCas8, VchCas7, VchCas6
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on Cas8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179051

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-RPC19-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PPP2R2B B6.5 gRNA

   Plasmid

   #90848

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PPP2R2B

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PPP2R2B (Guide Designation B6.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CHEK1 C1.2 gRNA

   Plasmid

   #90626

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CHEK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CHEK1 (Guide Designation C1.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_Anc689-nCas9

   Plasmid

   #163526

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAE1 G8.3 gRNA

    Plasmid

    #90777

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NAE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NAE1 (Guide Designation G8.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MSP2253

    Plasmid

    #70704

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAE1 G7.3 gRNA

    Plasmid

    #90776

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NAE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NAE1 (Guide Designation G7.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSDest2-NmeCas9-NLS-3XHA-NLS

    Plasmid

    #87448

    Purpose

    Mammalian expression of wild type Nme Cas9

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Nme Cas9
    

    Use

    CRISPR

    Tags

    3XHA, SV40 NLS, and cMyc-like NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid

    #157983

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458_KDM6A_1

    Plasmid

    #86307

    Purpose

    Encodes gRNA for 3' target of human KDM6A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM6A (KDM6A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDECKO_mCherry_CD19.4

    Plasmid

    #140101

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLC-EGFP-RIP1

    Plasmid

    #75163

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIPk1

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIPK1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB3051(gRNA X-3 XI-2 XII-2)

    Plasmid

    #73293

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

27. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PLK1 G5.1 gRNA

    Plasmid

    #90834

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK1 (Guide Designation G5.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6-sgGAL4-4

    Plasmid

    #46916

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-4
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109311

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Insert

    Ldlr gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179067

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-ERG6-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

    Plasmid

    #179066

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-ATG8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179060

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PIL1-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIK208

    Plasmid

    #65630

    Purpose

    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KIF2A D7.2 gRNA

    Plasmid

    #90720

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D7.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. KIF2A D8.2 gRNA

    Plasmid

    #90721

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D8.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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41. PSMD14 E6.4 gRNA

    Plasmid

    #90859

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PSMD14

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PSMD14 (Guide Designation E6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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42. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. PX458_FOXP1_1

    Plasmid

    #86299

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. ATAD2 D7.3 gRNA

    Plasmid

    #90538

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATAD2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ATAD2 (Guide Designation D7.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. PX458_KDM6A_2

    Plasmid

    #86308

    Purpose

    Encodes gRNA for 3' target of human KDM6A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM6A (KDM6A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. MSP2372

    Plasmid

    #73196

    Purpose

    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, and Q926A in SpCas9

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCD442

    Plasmid

    #153024

    Purpose

    dCas9, MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dCas9, MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. PX458_FOXP1_2

    Plasmid

    #86300

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge