171,784 results

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RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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AiP13028 - pAAV-AiE0461m_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3028)

Plasmid

#224022

Purpose

AiE0461m_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAL119-hCTLA4-Ig

Plasmid

#25100

Depositor

Maria Castro

Article

Guillot et al Blood. 2003 Apr 15. 101(8):3325-33.

Insert

ytotoxic T-murine Lymphocyte Antigen 4 (Ctla4 Mouse)

Use

Adenoviral

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-SAT1

Plasmid

#162465

Purpose

pFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNase

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

Plasmid

#124861

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mScarlet-TEV-Strep-His

Plasmid

#210823

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mScarlet

Tags

TwinStrep, 10xHis

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHygRR2r

Plasmid

#200076

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCri_L830-antiSH2-OptoMB-V416L

Plasmid

#159936

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Mutation

AsLOV2 V416L

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-G88P3-HA-hM3Dq

Plasmid

#213972

Purpose

AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

Depositor

Zhonghua Lu

Article

Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

Insert

hM3D (Gq)

Use

AAV

Promoter

G88P3

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

Plasmid

#156408

Purpose

pCS2 CMV expression vector driving EYFP fused human KRAS4B

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKS913.CB18.31

Plasmid

#69569

Purpose

TCR beta chain genomic transgenic construct for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

Insert

TCR beta (Tcrb Mouse)

Use

Mouse Targeting

Expression

Bacterial

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAG8H-EWS_1-264

Plasmid

#180467

Purpose

Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted 265-656

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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375 pME-fli1a

Plasmid

#49001

Purpose

full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1a (fli1 Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv4(pea3)

Plasmid

#49093

Purpose

expression of Etv4

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

Plasmid

#32135

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2RT> STOP STOP B2RT>

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192788

Purpose

aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody enhancer

Tags

H6 and ybbR

Expression

Bacterial

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-tdT

Plasmid

#59171

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

Plasmid

#194728

Purpose

AAVS1-tdTomato targeting vector, compartible with Cpf1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

tdTomato

Use

CRISPR; Knockin targeting plasmid

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cs2+Etv5

Plasmid

#49095

Purpose

expression of Etv5

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
R777-E206 Hs.PTEN-nostop

Plasmid

#70490

Purpose

Gateway ORF clone of human PTEN [NM_000314.5] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

PTEN (PTEN Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMNK56 - piRNAi 21ur-1224 - him-8

Plasmid

#159819

Purpose

piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

Depositor

Christian Froekjaer-Jensen

Article

Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

Insert

him-8 piRNAi 21ur-1224

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-4

Plasmid

#111474

Purpose

translational release factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 3 (Dll3 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pJJH1242

Plasmid

#36909

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-fli1b

Plasmid

#49019

Purpose

full length coding sequence for zebrafish fli1b gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1b (fli1rs Zebrafish)

Use

Gateway middle entry

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG458

Plasmid

#100900

Purpose

SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJH1307

Plasmid

#36910

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScLEU2

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TET-ZF37-mCherry-BGH_pKG01502

Plasmid

#246377

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TET-ZF43-TagBFP-BGH_pKG01531

Plasmid

#246378

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-minCMV)-mGL-BGH_pKG3654

Plasmid

#246333

Purpose

203-bp DIAL Reporter Plasmid with minCMV expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-IRES-Thy1.1-Aoah

Plasmid

#233141

Purpose

Thy1.1 and Aoah overexpression

Depositor

Peng Jiang

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl hydrolase (Aoah Mouse)

Use

Lentiviral

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15256 - pAAV-AiE0664m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5256)

Plasmid

#229931

Purpose

AiE0664m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-BUBR1

Plasmid

#234704

Purpose

expression of fusion protein

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

BUBR1 (BUB1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

Plasmid

#235608

Purpose

Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms

Use

CRISPR

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459_L31_CRISPR_downstream_sgRNA

Plasmid

#235610

Purpose

Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus downstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459_L31_CRISPR_upstream_sgRNA

Plasmid

#235609

Purpose

Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus upstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1A-HALO-hLRRK2

Plasmid

#229738

Purpose

Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1318 - pAAV-AiE2013m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230527

Purpose

AiE2013m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12582 - pAAV-AiE0486m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2582)

Plasmid

#229894

Purpose

AiE0486m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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