4,170 results

1. pRL623

   Plasmid

   #58494

   Purpose

   helper plasmid for bacterial conjugal DNA transfer

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Insert

   encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
   

   Available Since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

   Plasmid

   #190163

   Purpose

   Microglia-targeted transgene expression.

   Depositor

   Hirokazu Hirai

   Article

   Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

   Inserts

   mIba1 promoter, 1.7-kb, microglia-specific promoter
   miR-9-Tx4, 4 repeated miRNA target sites for miR-9
   miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
   

   Use

   AAV

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV rtTA3 Hygro (w785-1)

   Plasmid

   #26730

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLKO.1 puro shRNA beta-catenin

   Plasmid

   #18803

   Purpose

   3rd gen lentiviral vector for knocking down beta-catenin gene expression

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   b-catenin shRNA (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAS-luc2

   Plasmid

   #24343

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   Luciferase
   

   Tags

   5XGAL4

   Expression

   Mammalian

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIRT2 Flag

    Plasmid

    #13813

    Purpose

    Mammalian expression of human SIRT2 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT2 (SIRT2 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Transcript variant 2

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-zeo largeTcDNA

    Plasmid

    #1779

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEx-ER-TurboID

    Plasmid

    #160857

    Purpose

    Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Has Service

    AAV8

    Insert

    ER TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Promoter

    CAG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnCasPA-BEC

    Plasmid

    #113349

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBtet-Pur

    Plasmid

    #60507

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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