174,131 results

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pmTurquoise2-Peroxi

Plasmid

#36203

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Peroxisomal targeting sequence

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-HA (New MCS)

Plasmid

#32530

Depositor

Christopher A Walsh

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-p53

Plasmid

#22725

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-sox2

Plasmid

#20326

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

sox2 (Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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EF.hHES1.Ubc.GFP

Plasmid

#17624

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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GFP::L4440

Plasmid

#11335

Depositor

Guy Caldwell

Insert

GFP

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pBS aP2 promoter (5.4kb) polyA

Plasmid

#11424

Depositor

Ronald Kahn

Insert

aP2 promoter (Fabp4 Mouse)

Expression

Bacterial

Available Since

April 3, 2006

Availability

Academic Institutions and Nonprofits only

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1245 pCMVb p300

Plasmid

#10717

Depositor

William Sellers

Insert

p300 (EP300 Human)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-mNeonGreen

Plasmid

#188115

Purpose

Template plasmid to transcribe circular RNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Article

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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PBCAG-eGFP

Plasmid

#40973

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Inserts

PiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats

Expression

Mammalian

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SuperNova/pRSETB

Plasmid

#53234

Purpose

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Takeharu Nagai

Article

Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

Insert

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Article

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 with human c-terminus, Rat)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

Plasmid

#28289

Purpose

3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

Depositor

Alex Chenchik, Gus Frangou

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-cortactin

Plasmid

#26722

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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XBP-1 1: pFLAG.XBP1u.CMV2

Plasmid

#21832

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 unprocessed (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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EF.hICN1.Ubc.GFP

Plasmid

#17626

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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HA-Foxo1 (pCMV5)

Plasmid

#12142

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 (Foxo1 Mouse)

Tags

HA and Myc

Expression

Mammalian

Mutation

Wild-type

Promoter

CMV

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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