We narrowed to 9,389 results for: Coli

1. pKIKOlacZCm

   Plasmid

   #46764

   Purpose

   E.coli genomic integration at lacZ

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJM.011

   Plasmid

   #108529

   Purpose

   TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   TetR + PTet*-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKIKOlacZKm

   Plasmid

   #46767

   Purpose

   E.coli genomic integration at lacZ

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6his-WT gusA-pET28a+

   Plasmid

   #61156

   Purpose

   N -terminal His tagged wild-type GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   gusA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p006-GFP-pBAD

   Plasmid

   #108315

   Purpose

   GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   GFP under control of arabinose-inducible promoter pBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PT7lacO-EF-Ts-His6

   Plasmid

   #224371

   Purpose

   Express EF-Ts protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-Ts (tsf E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PT7lacO-EF-G-His8

   Plasmid

   #224369

   Purpose

   Express EF-G protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-G (fusA E. coli)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PT7lacO-His6-PheRSa-PheRSb

   Plasmid

   #224364

   Purpose

   Express PheRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   PheRS (pheS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PT7lacO-His6-AlaRS

   Plasmid

   #224362

   Purpose

   Express AlaRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AlaRS (alaS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPhlF-P3

    Plasmid

    #74687

    Purpose

    PhlF-P3 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSP3-PhlF-PPhlF-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP E17TAG

    Plasmid

    #68295

    Purpose

    E17TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCWori-A13AMO-aaCPRct

    Plasmid

    #20117

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    Amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKIKOrbsARCm

    Plasmid

    #46765

    Purpose

    E.coli genomic integration rbsAR locus

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKIKOrbsARKm

    Plasmid

    #46768

    Purpose

    E.coli genomic integration rbsAR locus

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p177bp-LacOx162-Hyg

    Plasmid

    #210215

    Purpose

    Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    162xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW071

    Plasmid

    #140037

    Purpose

    PCC 11901 fadD locus KO with a kanamycin resistance cassette

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Kanamycin resistance gene
    

    Use

    Gene knockout in cyanobacteria

    Tags

    Expression

    Mutation

    Promoter

    KanR

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBbE1k-DDC

    Plasmid

    #71723

    Purpose

    dopa decarboxylase from pig

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Insert

    dopa decarboxylase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. RP4-7

    Plasmid

    #79811

    Purpose

    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Own promoter

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pBEL1994

    Plasmid

    #139898

    Purpose

    Expression plasmid for LetB PLL2 deletion (without TM region)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta229-239, D228G, L240G)-6xHis

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMA3442

    Plasmid

    #184852

    Purpose

    Retroviral vector encoding puromycin resistance and Cre recombinase

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJMP8045

    Plasmid

    #220900

    Purpose

    Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKSJ331

    Plasmid

    #103063

    Purpose

    Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

    Depositor

    Karen Jakes, Alan Finkelstein

    Article

    Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

    Insert

    Colicin E1 and E1 immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    colicin promotor from ColE1

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p006-GFP-weak

    Plasmid

    #108314

    Purpose

    GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4282

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBLind

    Plasmid

    #113109

    Purpose

    Vector encoding the RFP gene under blue light-inducible promoter BindF1.

    Depositor

    Chueh Loo Poh

    Article

    Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

    Insert

    RFP
    

    Use

    Cell-free

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BindF1

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-AqpZ-ALFA

    Plasmid

    #212310

    Purpose

    Expression of AqpZ with ALFA tag

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    Aquaporin Z
    

    Use

    Tags

    ALFA tag and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    metY native promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLS.436

    Plasmid

    #184953

    Purpose

    Express -Eco1 RT and ncRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: ncRNA(wt) and RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAJM.715

    Plasmid

    #108516

    Purpose

    PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJYP1

    Plasmid

    #202406

    Purpose

    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

    Depositor

    Remy Loris

    Article

    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    fdeA

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CAG.mCherry-eDHFR

    Plasmid

    #176201

    Purpose

    Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-eDHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEF/myc/ER FLII12Pglu-700uDelta6

    Plasmid

    #17867

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLII12Pglu-700uDelta6
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    700uDelta6, ECFP insert in mglB

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge