59,446 results

1. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-mCherry-FTH1

   Plasmid

   #100749

   Purpose

   Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TfR-pHuji

   Plasmid

   #61505

   Purpose

   Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

   Depositor

   David Perrais

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   Transferrin receptor (TFRC Human)
   

   Tags

   pHuji

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5B-TGFbeta receptor II wt (N-term HA)

   Plasmid

   #24801

   Depositor

   Jeff Wrana

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. mNeonGreen-KDEL

   Plasmid

   #226568

   Purpose

   Encodes mNeonGreen with KDEL ER lumenal target sequence

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   KDEL (ER lumenal target sequence)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v6 (fl)

   Plasmid

   #137816

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R296K in the V6 region [Please see depositor comm…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLCAR-CD19-CD3z

   Plasmid

   #135993

   Purpose

   Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-3z-P2A-EGFP (CD247, CD28 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-UBE2E1 WT in pcDNA3.1

   Plasmid

   #118213

   Purpose

   Expresses HA-UBE2E1 in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2815

    Plasmid

    #144244

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOSB (FOSB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104906

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HPSE-bio-His

    Plasmid

    #53407

    Purpose

    Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    HPSE (HPSE Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-SREBP-1-GFP (A317-318; A341)

    Plasmid

    #204198

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Tags

    3xHA, eGFP and T7x2

    Expression

    Mammalian

    Mutation

    Gly to Ala 317,318,341

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNAKv10.1mVenus

    Plasmid

    #85705

    Purpose

    Kv10.1 fused mVenus

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-hSTING (N154S)

    Plasmid

    #214160

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N154S) (STING1 Human)
    

    Expression

    Mammalian

    Mutation

    N154S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

    Plasmid

    #86885

    Purpose

    C-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    BirA* (AA141-321)- HA- NIPP1]
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 141-321

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF3192

    Plasmid

    #144668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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