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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_PCP-T5Exo

Plasmid

#214736

Purpose

encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

Depositor

Gil Westmeyer

Article

Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

Insert

CAG_PCP-T5Exo_bGH

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn3L-mNeonGreen

Plasmid

#169034

Purpose

Express human Rtn3L in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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Human Parkin (1-465)

Plasmid

#110758

Purpose

Bacterial expression of human Parkin

Depositor

David Komander

Article

Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

Insert

PRKN (PRKN Human)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Klotho (secreted)

Plasmid

#17713

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

His and V5

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP TMEM41B-GFP

Plasmid

#117414

Purpose

Expresses TMEM41B tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple (bidirectional) GasS

Plasmid

#196055

Purpose

Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphasS-RLuc8
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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SunTagng22aa

Plasmid

#106438

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2962

Plasmid

#144438

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR2C2 (NR2C2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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BCL2alpha-MJK2243

Plasmid

#227690

Purpose

Expresses human BCL2alpha

Depositor

Curt Civin

Article

Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

Insert

human BCL-2alpha (BCL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR-v1-sgGLS_2

Plasmid

#163459

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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